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Invitrogen
CD81 Recombinant Mouse Monoclonal Antibody (1.3.3.22)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 1
CD81 Antibody (MA5-48280)
Antibody clone (1.3.3.22) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD81 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD81 antibody (1.3.3.22). Yellow histogram represents staining with the isotype control. {KO}
Product Details
MA5-48280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1.3.3.22
Immunogen
This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074722
Product Specific Information
Specificity: 1.3.3.22 antibody recognizes CD81. Human CD81 is a member of the tetraspanin family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. It is present on a wide variety of leukocytes.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28
Gene Aliases: CD81; CVID6; S5.7; Tapa-1; TAPA1; TSPAN28
UniProt ID: (Human) P60033, (Mouse) P35762
Entrez Gene ID: (Human) 975, (Rat) 25621, (Mouse) 12520
activation of MAPK activity
phosphatidylinositol biosynthetic process
protein localization
positive regulation of cell proliferation
response to wounding
positive regulation of B cell proliferation
regulation of growth
regulation of cell proliferation
positive regulation of 1-phosphatidylinositol 4-kinase activity
phosphatidylinositol metabolic process
positive regulation of peptidyl-tyrosine phosphorylation
regulation of cell motility
cell surface receptor signaling pathway
cell proliferation
receptor internalization
regulation of protein stability
positive regulation of transcription from RNA polymerase II promoter
viral entry into host cell
receptor-mediated virion attachment to host cell
regulation of immune response
protein localization to lysosome
cellular response to low-density lipoprotein particle stimulus
positive regulation of protein catabolic process in the vacuole
positive regulation of cell growth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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