Invitrogen

CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (66) CD81 antibodies

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CD81 Antibody (17-0819-42) in Flow

Knockout of CD81 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR661098_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD81 was performed by staining THP-1 CD81 Knock out cells with 1 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ (Product # 17-4714-82, yellow histogram) or 1 µg CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™ (Product # 17-0819-42, blue histogram). THP-1 Cas9 control cells were also stained with1 µ... View More

Product Image Gallery

CD81 Antibody — Antibody clone (1D6-CD81) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 1D6-CD81 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD81 antibody (1D6-CD81). Yellow histogram represents staining with the isotype control. {KO}

Product Details

17-0819-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1D6-CD81

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11150235

Product Specific Information

Description: This 1D6-CD81 monoclonal antibody reacts with human CD81, which is also known as Target of Anti-Proliferative Ab-1 (TAPA-1). A member of the tetraspanin superfamily of transmembrane proteins, CD81 is widely expressed on immune cells such as B, T, and NK cells, monocytes, and eosinophils. Studies suggest that the highest expression of CD81 can be found on germinal center B cells. This protein can also be detected on non-Hodgkin lymphomas and diffuse large B-cell lymphomas. On B cells, CD81 exists in a complex with CD19, CD21, and Leu13. CD81 plays a role in segregating the CD19/CD21-B cell receptor complexes to lipid rafts to activate signal transduction. Finally, CD81 is a receptor for hepatitis C virus.

The 1D6 monoclonal antibody has been reported to induce adhesion and reduce cell proliferation.

Applications Reported: This 1D6-CD81 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1D6-CD81 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD81 monoclonal antibody (Product # 17-0819-42) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Insights into cellular behavior and micromolecular communication in urothelial micrografts.

Scientific reports

Juul N,Willacy O,Mamand DR,Andaloussi SE,Eisfeldt J,Chamorro CI,Fossum M

17-0819-42 was used in Flow Cytometry to demonstrate that increased tissue fragmentation subsequently potentiated the yield of proliferative cells and the cellular expansion potential, which confirms, that the micrografting principles of skin epithelium also apply to uroepithelium.

Mon Aug 21 00:00:00 EDT 2023

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Sat May 01 00:00:00 EDT 2021

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Sat May 01 00:00:00 EDT 2021

Open conformation of tetraspanins shapes interaction partner networks on cell membranes.

The EMBO journal

Yang Y,Liu XR,Greenberg ZJ,Zhou F,He P,Fan L,Liu S,Shen G,Egawa T,Gross ML,Schuettpelz LG,Li W

17-0819-42 was used in Western Blotting to investigate if an open conformation is required for tetraspanin-partner interactions to support myriad cellular processes.

Tue Sep 15 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells.

The EMBO journal

He X,Kläsener K,Iype JM,Becker M,Maity PC,Cavallari M,Nielsen PJ,Yang J,Reth M

17-0819 was used in Flow cytometry/Cell sorting to show that Igβ and CD19 are part of an alternative B-cell signalling module that use continuous ITAM/PI3K signalling to promote the survival of B lymphoma and normal B cells.

Fri Jun 01 00:00:00 EDT 2018

CD81 protein is expressed at high levels in normal germinal center B cells and in subtypes of human lymphomas.

Human pathology

Luo RF,Zhao S,Tibshirani R,Myklebust JH,Sanyal M,Fernandez R,Gratzinger D,Marinelli RJ,Lu ZS,Wong A,Levy R,Levy S,Natkunam Y

Published figure using CD81 monoclonal antibody (Product # 17-0819-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: 26 kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28

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UniProt ID: (Human) P60033

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Entrez Gene ID: (Human) 975

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Function(s)

protein binding MHC class II protein complex binding transferrin receptor binding

Process(es)

activation of MAPK activity cell surface receptor signaling pathway protein localization cell proliferation positive regulation of cell proliferation response to wounding positive regulation of B cell proliferation receptor internalization regulation of protein stability regulation of growth positive regulation of 1-phosphatidylinositol 4-kinase activity positive regulation of transcription from RNA polymerase II promoter viral entry into host cell receptor-mediated virion attachment to host cell positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response protein localization to lysosome cellular response to low-density lipoprotein particle stimulus positive regulation of protein catabolic process in the vacuole regulation of cell motility

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CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™

CD81 Antibody (17-0819-42) in Flow

Product Details

17-0819-42

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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