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Invitrogen
CD81 Monoclonal Antibody (M38)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
CD81 Antibody (MA5-44066) in WB
Western blotting analysis of human CD81 using mouse Monoclonal antibody M38 (Product # MA5-44066) on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/mL of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.
Product Details
MA5-44066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Human,
Rabbit
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M38
Immunogen
MOLT-4 (human T-ALL cell line)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912998
Product Specific Information
This antibody reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Positive control: Jurkat cells, non-reducing conditions.
Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26 kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28
Gene Aliases: CD81; CVID6; S5.7; TAPA1; TSPAN28
UniProt ID: (Human) P60033
Entrez Gene ID: (Human) 975
activation of MAPK activity
cell surface receptor signaling pathway
protein localization
cell proliferation
positive regulation of cell proliferation
response to wounding
positive regulation of B cell proliferation
receptor internalization
regulation of protein stability
regulation of growth
positive regulation of 1-phosphatidylinositol 4-kinase activity
positive regulation of transcription from RNA polymerase II promoter
viral entry into host cell
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
protein localization to lysosome
cellular response to low-density lipoprotein particle stimulus
positive regulation of protein catabolic process in the vacuole
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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