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Product Details
MA5-46740
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
105
Immunogen
Recombinant protein ecoding Human CD83 (Met1-Ala143).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937812
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD83 (Met1-Ala143) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell activation protein; CD antigen CD83; CD83; CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); Cell surface protein HB15; cell-surface glycoprotein; hCD83
Gene Aliases: BL11; CD83; HB15
UniProt ID: (Human) Q01151
Entrez Gene ID: (Human) 9308
regulation of cytokine production
defense response
humoral immune response
signal transduction
response to organic cyclic compound
negative regulation of interleukin-4 production
positive regulation of interleukin-10 production
positive regulation of interleukin-2 production
positive regulation of CD4-positive, alpha-beta T cell differentiation
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