Invitrogen

CD83 Monoclonal Antibody (Michel-17 (Michel17)), eBioscience™

FIGURE: 1 / 4

CD83 Antibody (14-0831-82) in Flow

Staining of 3-day unstimulated (left) and 3-day LPS-stimulated (right) BALB/c splenocytes with 0.25 µg of Rat Ig1 K Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD83 Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

14-0831-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 10 publications 10 publications

Mass Spectrometry (MS)

- -

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

Michel-17 (Michel17)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467357

Product Specific Information

Description: The Michel-17 monoclonal antibody reacts with mouse CD83, a 45kDa cell surface glycoprotein and a member of the Ig superfamily. The mouse CD83 antigen is expressed predominantly on mature DC and activated lymphocytes. Cross-linking of CD83 with Michel-17 on DC or activated T cells does not induce any activation signal. CD83 plays an important role in T cell development through interaction with its ligand. CD83-Ig protein has revealed the presence of a CD83 ligand expressed mainly by B220^+ cells in mouse spleen.

Applications Reported: The Michel-17 (Michel17) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Michel-17 (Michel17) antibody has been tested by flow cytometric analysis of LPS activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model.

Scientific reports

Wang H,Mo L,Xiao X,An S,Liu X,Ba J,Wu W,Ran P,Yang P,Liu Z

Published figure using CD83 monoclonal antibody (Product # 14-0831-82) in Flow Cytometry

Mon Feb 27 00:00:00 EST 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using CD83 monoclonal antibody (Product # 14-0831-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using CD83 monoclonal antibody (Product # 14-0831-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

The Polyunsaturated Fatty Acids Arachidonic Acid and Docosahexaenoic Acid Induce Mouse Dendritic Cells Maturation but Reduce T-Cell Responses In Vitro.

PloS one

Carlsson JA,Wold AE,Sandberg AS,Östman SM

14-0831 was used in Blocking experiments to measure the effects of omega-3, -6 and -9 fatty acids on the interaction between dendritic cells and naïve T cells.

Fri Jul 01 00:00:00 EDT 2016

Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function.

Parasite immunology

Wanderley JLM,Thorpe PE,Barcinski MA,Soong L

14-0831 was used in Flow cytometry/Cell sorting to provide new information regarding the mechanism of immune suppression in Leishmania infection.

Wed Jul 17 00:00:00 EDT 2013

Cytokine therapy with interleukin-2/anti-interleukin-2 monoclonal antibody complexes expands CD4+CD25+Foxp3+ regulatory T cells and attenuates development and progression of atherosclerosis.

Circulation

Dinh TN,Kyaw TS,Kanellakis P,To K,Tipping P,Toh BH,Bobik A,Agrotis A

14-0831 was used in Immunohistochemistry to examine the capacity of a modified IL-2/anti-IL-2 monoclonal antibody treatment to expand Tregs and attenuate atherosclerosis in mice.

Tue Sep 04 00:00:00 EDT 2012

Depletion of B2 but not B1a B cells in BAFF receptor-deficient ApoE mice attenuates atherosclerosis by potently ameliorating arterial inflammation.

PloS one

Kyaw T,Tay C,Hosseini H,Kanellakis P,Gadowski T,MacKay F,Tipping P,Bobik A,Toh BH

14-0831 was used in Immunohistochemistry to demonstrate that B2 cells are important contributors to the development of atherosclerosis, and to determine the mechanism of contribution.

Mon May 14 00:00:00 EDT 2012

CD83 increases MHC II and CD86 on dendritic cells by opposing IL-10-driven MARCH1-mediated ubiquitination and degradation.

The Journal of experimental medicine

Tze LE,Horikawa K,Domaschenz H,Howard DR,Roots CM,Rigby RJ,Way DA,Ohmura-Hoshino M,Ishido S,Andoniou CE,Degli-Esposti MA,Goodnow CC

14-0831 was used in Flow cytometry/Cell sorting to investigate how CD83 expressed on the surface of mature dendritic cells promotes MHC class II and CD86 expression.

Mon Jan 17 00:00:00 EST 2011

High-mobility group box protein 1 neutralization reduces development of diet-induced atherosclerosis in apolipoprotein e-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Kanellakis P,Agrotis A,Kyaw TS,Koulis C,Ahrens I,Mori S,Takahashi HK,Liu K,Peter K,Nishibori M,Bobik A

14-0831 was used in Immunohistochemistry to reveal that HMGB1 augments atherosclerotic lesion development by stimulating macrophage migration and modulating proinflammatory mediators, and represents a potential therapeutic target for atherosclerosis.

Tue Feb 01 00:00:00 EST 2011

Comparison of gene expression profiles between human and mouse monocyte subsets.

Blood

Ingersoll MA,Spanbroek R,Lottaz C,Gautier EL,Frankenberger M,Hoffmann R,Lang R,Haniffa M,Collin M,Tacke F,Habenicht AJ,Ziegler-Heitbrock L,Randolph GJ

14-0831 was used in Flow cytometry/Cell sorting to identify how human and mouse monocyte subsets are far more broadly conserved than currently recognised.

Thu Jan 21 00:00:00 EST 2010

Lethal effect of CD3-specific antibody in mice deficient in TGF-beta1 by uncontrolled flu-like syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Perruche S,Zhang P,Maruyama T,Bluestone JA,Saas P,Chen W

14-0831 was used in Flow cytometry/Cell sorting to indicate a key role for TGF-beta1 and phagocytes in protecting recipients from lethal inflammation after CD3-specific Ab treatment.

Wed Jul 15 00:00:00 EDT 2009

17beta-Estradiol's salutary effects on splenic dendritic cell functions following trauma-hemorrhage are mediated via estrogen receptor-alpha.

Molecular immunology

Kawasaki T,Choudhry MA,Suzuki T,Schwacha MG,Bland KI,Chaudry IH

14-0831 was used in Flow cytometry/Cell sorting to investigate the effect of 17beta-estradiol on splenic dendritic cell function, showing that beneficial effects occur via estrogen receptor-alpha.

Tue Jan 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CD antigen CD83; CD83; CD83 antigen; mCD83

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Gene Aliases: Cd83

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UniProt ID: (Mouse) O88324

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Entrez Gene ID: (Mouse) 12522

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Process(es)

response to organic cyclic compound negative regulation of interleukin-4 production positive regulation of interleukin-10 production positive regulation of interleukin-2 production positive regulation of CD4-positive, alpha-beta T cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD83 Monoclonal Antibody (Michel-17 (Michel17)), eBioscience™

CD83 Antibody (14-0831-82) in Flow

Product Details

14-0831-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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