Invitrogen

CD86 Monoclonal Antibody (BU63)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

34 References

View all (188) CD86 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FIGURE: 1 / 6

CD86 Antibody (MA1-10293) in Flow

Flow Cytometry analysis of human Raji cells (left panel) and mouse spenocytes (right panel) using CD86 monoclonal antibody (Product # MA1-10293) at 0.5µg x 10^6 cells in 100µL. CD86 (green) was detected using a DyLight-488 Donkey anti-Mouse IgG (H+L) cross-adsorbed secondary antibody (Product # A-21202) at 1:200. The negative control (blue) represents Mouse IgG Isotype Control (Product # MA5-14453). Results show positive staining in human cells with no cross-reactivity to mouse. Data provided courtesy of Antibody Resource.

CD86 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

CD86 Antibody — Figure 1 HF-LPLI promoted neutrophil apoptosis and MPhi reprogramming, and inhibited the release of inflammatory factors. (A) Apoptosis of HF-LPLI-treated neutrophils was detected by flow cytometry. (B) Apoptosis activation in neutrophils, detected by caspase-3 activity assay. (C) CD86 and CD163 immunofluo-rescent staining using red and green fluorophores, respectively, and nuclei stained with DAPI (scale bar, 100 mu m). (D) Bar graph of CD86- and CD163-positive MPhi percentage by immunofluorescent staining. (E) NO concentration examined by DAF-FM DA. (F) Concentration of inflammatory factors, including TNF-alpha, IL-6, IL-10 and TGF-beta, determined by ELISA. The results are representative of 10 independent experiments, and are expressed as the mean +- standard deviation. **P<0.01 vs. LPS group (n=10). HF-LPLI, high-frequency low-power laser irradiation; MPhi, macrophage; TNF-alpha, tumor necrosis factor alpha; IL, interleukin; TGF-beta, transforming growth factor-beta; LPS, lipopolysaccharide. {TM}

Product Details

MA1-10293

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µg/ml

View 23 publications 23 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BU63

Immunogen

B-lymphoblastoid cell line ARH 77

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153536

Product Specific Information

This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High-fluence low-power laser irradiation promotes odontogenesis and inflammation resolution in periodontitis by enhancing stem cell proliferation and differentiation.

International journal of molecular medicine

Hou T,Li S,Zhang G,Li Y

Published figure using CD86 monoclonal antibody (Product # MA1-10293) in Immunofluorescence

Mon Oct 01 00:00:00 EDT 2018

Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.

Toxicology in vitro : an international journal published in association with BIBRA

Mytych J,Romerowicz-Misielak M,Koziorowski M

MA110293 was used in western blot to report the effects of long-term lipopolysaccharide exposure on monocytes

Tue Aug 01 00:00:00 EDT 2017

A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.

Retrovirology

Babu R,Brown A

MA1-10293 was used in flow cytometry to explore the role of HIV in potentially modulating macrophage function

Mon Dec 16 00:00:00 EST 2013

Human follicular dendritic cells promote the APC capability of B cells by enhancing CD86 expression levels.

Cellular immunology

Kim J,Kim YM,Jeoung DI,Choe J

MA1-10293 was used in flow cytometry to test if follicular dendritic cells contribute to the antigen-presenting capability of B cells.

Thu Apr 12 00:00:00 EDT 2012

Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.

Human immunology

Garbe K,Bratke K,Wagner S,Virchow JC,Lommatzsch M

MA1-10293 was used in flow cytometry to examine the effects of age and gender on TLR9 expression on human DCs.

Tue May 01 00:00:00 EDT 2012

HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation.

PloS one

St Gelais C,Coleman CM,Wang JH,Wu L

MA1-10293 was used in flow cytometry to determine the role of HIV-1 Nef in dendritic cell-mediated viral transmission and HIV-1 infection

Mon Jul 30 00:00:00 EDT 2012

Human dendritic cell culture and bacterial infection.

Methods in molecular biology (Clifton, N.J.)

Jones HE,Klein N,Dixon GL

MA1-10293 was used in flow cytometry to discuss methods to generate human monocyte-derived DCs and assess their maturation, activation, and function, using interaction with the gram-negative bacterial pathogen Neisseria meningitidis as a model.

Wed Feb 01 00:00:00 EST 2012

Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef.

Retrovirology

Coleman CM,Spearman P,Wu L

MA1-10293 was used in flow cytometry to investigate the role of type I IFN and tetherin in HIV-1 infection of dendritic cells

Tue Apr 19 00:00:00 EDT 2011

Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.

Arthritis and rheumatism

Morbach H,Wiegering V,Richl P,Schwarz T,Suffa N,Eichhorn EM,Eyrich M,Girschick HJ

MA1-10293 was used in Flow cytometry/Cell sorting to investigate whether synovial B cells in juvenile idiopathic arthritis display features of antigen-presenting cells.

Tue Nov 01 00:00:00 EDT 2011

Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.

Arthritis and rheumatism

Morbach H,Wiegering V,Richl P,Schwarz T,Suffa N,Eichhorn EM,Eyrich M,Girschick HJ

MA1-10293 was used in Flow cytometry/Cell sorting to investigate whether synovial B cells in juvenile idiopathic arthritis display features of antigen-presenting cells.

Tue Nov 01 00:00:00 EDT 2011

The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Schrauf C,Kirchberger S,Majdic O,Seyerl M,Zlabinger GJ,Stuhlmeier KM,Sachet M,Seipelt J,Stöckl J

MA1-10293 was used in flow cytometry to study the effect of natural occurring viral ssRNA on DC function

Thu Oct 01 00:00:00 EDT 2009

HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis.

Arthritis and rheumatism

Kuhne M,Erben U,Schulze-Tanzil G,Köhler D,Wu P,Richter FJ,John T,Radbruch A,Sieper J,Appel H

MA1-10293 was used in flow cytometry to test if chondrocytes attract lymphocytes and if cartilage chondrocytes from patients with ankylosing spondylitis stimulate T cells in an HLA-restricted manner

Mon Jun 01 00:00:00 EDT 2009

Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.

Journal of immunology (Baltimore, Md. : 1950)

Stephens TA,Nikoopour E,Rider BJ,Leon-Ponte M,Chau TA,Mikolajczak S,Chaturvedi P,Lee-Chan E,Flavell RA,Haeryfar SM,Madrenas J,Singh B

MA1-10293 was used in flow cytometry to identify and characterize a signal transduction cascade in monocytes that results in their differentiation into dendritic cells

Sat Nov 15 00:00:00 EST 2008

Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95.

Arthritis and rheumatism

Jacobi AM,Reiter K,Mackay M,Aranow C,Hiepe F,Radbruch A,Hansen A,Burmester GR,Diamond B,Lipsky PE,Dörner T

MA1-10293 was used in flow cytometry to identify changes in peripheral B cell subsets in patients with systemic lupus erythematosus

Sun Jun 01 00:00:00 EDT 2008

A novel human B cell subpopulation representing the initial germinal center population to express AID.

Blood

Kolar GR,Mehta D,Pelayo R,Capra JD

MA1-10293 was used in flow cytometry to characterize B cells in human tonsils

Thu Mar 15 00:00:00 EDT 2007

Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.

Clinical immunology (Orlando, Fla.)

Summers KL,Marleau AM,Mahon JL,McManus R,Hramiak I,Singh B

MA1-10293 was used in flow cytometry to characterize dendritic cells in type 1 and type 2 diabetes

Sun Oct 01 00:00:00 EDT 2006

Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.

Immunology

Sandilands GP,McCrae J,Hill K,Perry M,Baxter D

MA1-10293 was used in flow cytometry and immunocytochemistry to compare X-L of Mac -1 with other neutrophil activators

Fri Dec 01 00:00:00 EST 2006

Human monocyte-derived dendritic cells are a source of several complement proteins.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Reis ES,Barbuto JA,Isaac L

MA1-10293 was used in flow cytometry to show that human dendritic cells are an extrahepatic source of soluble complement proteins

Mon May 01 00:00:00 EDT 2006

Expression of CD45 and proliferative response to CD3 as suitable classification markers of patients with gastric adenocarcinoma.

Cancer immunology, immunotherapy : CII

López-Santalla M,Valeri AP,Pérez-Blas M,Aguilera-Montilla N,Gutiérrez A,Lasa I,Mugüerza JM,Martín J,García-Sancho L,Granell J,Martín-Villa JM

MA1-10293 was used in flow cytometry to characterize gastric adenocarcinoma with and without residual neoplasic disease

Thu Jun 01 00:00:00 EDT 2006

Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Game DS,Rogers NJ,Lechler RI

MA1-10293 was used in flow cytometry to test if transfer of allogeneic material to T cells modifies human alloresponses

Fri Jul 01 00:00:00 EDT 2005

Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation.

Immunology

Sandilands GP,Ahmed Z,Perry N,Davison M,Lupton A,Young B

MA1-10293 was used in flow cytometry to investigate how neutrophils present antigen

Tue Mar 01 00:00:00 EST 2005

Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV-infected immature dendritic cells.

European journal of immunology

Lozza L,Lilleri D,Percivalle E,Fornara C,Comolli G,Revello MG,Gerna G

MA1-10293 was used in flow cytometry to study human cytomegalovirus-specific CD4+ and CD8+ T cells

Wed Jun 01 00:00:00 EDT 2005

Profiling dendritic cell maturation with dedicated microarrays.

Journal of leukocyte biology

McIlroy D,Tanguy-Royer S,Le Meur N,Guisle I,Royer PJ,Léger J,Meflah K,Grégoire M

MA1-10293 was used in flow cytometry to develop a dendritic cell-dedicated microarray

Thu Sep 01 00:00:00 EDT 2005

Oxidized phospholipids negatively regulate dendritic cell maturation induced by TLRs and CD40.

Journal of immunology (Baltimore, Md. : 1950)

Blüml S,Kirchberger S,Bochkov VN,Krönke G,Stuhlmeier K,Majdic O,Zlabinger GJ,Knapp W,Binder BR,Stöckl J,Leitinger N

MA1-10293 was used in flow cytometry to show that oxidized phospholipid treatment interferes with dendritic cell maturation

Fri Jul 01 00:00:00 EDT 2005

Bacterial ghosts as novel efficient targeting vehicles for DNA delivery to the human monocyte-derived dendritic cells.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Kudela P,Paukner S,Mayr UB,Cholujova D,Schwarczova Z,Sedlak J,Bizik J,Lubitz W

MA1-10293 was used in flow cytometry to report that bacterial ghosts can deliver nucleic acid-encoded antigens

Tue Jun 28 00:00:00 EDT 2005

Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.

Journal of immunology (Baltimore, Md. : 1950)

Zingoni A,Sornasse T,Cocks BG,Tanaka Y,Santoni A,Lanier LL

MA1-10293 was used in flow cytometry to report that OX40 ligand is selectively induced by IL-2, IL-12, or IL-15-activated human NK cells following stimulation through NKG2D

Wed Sep 15 00:00:00 EDT 2004

CD63 as an activation-linked T cell costimulatory element.

Journal of immunology (Baltimore, Md. : 1950)

Pfistershammer K,Majdic O,Stöckl J,Zlabinger G,Kirchberger S,Steinberger P,Knapp W

MA1-10293 was used in flow cytometry to elucidate how the monoclonal antibody, 11C9, reduces dendritic cell-induced T cell activation

Mon Nov 15 00:00:00 EST 2004

Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines.

Scandinavian journal of gastroenterology

Aguilera-Montilla N,Pérez-Blas M,Valeri AP,López-Santalla M,Rodríguez-Juan C,Mencía A,Castellano G,Manzano ML,Casis B,Sánchez F,Martín-Villa JM

MA1-10293 was used in flow cytometry to discuss the creation of stable T-cell lines to study their impact in inflammatory bowel disease

Wed Dec 01 00:00:00 EST 2004

White monkey syndrome in infant baboons (Papio species).

Journal of medical primatology

Frost PA,Hubbard GB,Dammann MJ,Snider CL,Moore CM,Hodara VL,Giavedoni LD,Rohwer R,Mahaney MC,Butler TM,Cummins LB,McDonald TJ,Nathanielsz PW,Schlabritz-Loutsevitch NE

MA1-10293 was used in flow cytometry to describe the epidemiologic and clinical presentation of zinc toxicosis in baboons in captivity

Sun Aug 01 00:00:00 EDT 2004

Intrinsic defects explain altered proliferative responses of T lymphocytes and HVS-derived T-cell lines in gastric adenocarcinoma.

Cancer immunology, immunotherapy : CII

Valeri AP,Pérez-Blas M,Gutiérrez A,López-Santalla M,Aguilera N,Rodríguez-Juan C,Sala-Silveira L,Martín J,Lasa I,Mugüerza JM,López A,García-Sancho L,Granell J,Martín-Villa JM

MA1-10293 was used in flow cytometry to study T-cell lines from gastric cancer patients and healthy volunteers

Sat Nov 01 00:00:00 EST 2003

B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Selenko-Gebauer N,Majdic O,Szekeres A,Höfler G,Guthann E,Korthäuer U,Zlabinger G,Steinberger P,Pickl WF,Stockinger H,Knapp W,Stöckl J

MA1-10293 was used in ELISA to identify immunoregulatory molecules on dendritic cells.

Tue Apr 01 00:00:00 EST 2003

Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning.

Journal of leukocyte biology

Steinberger P,Szekeres A,Wille S,Stöckl J,Selenko N,Prager E,Staffler G,Madic O,Stockinger H,Knapp W

MA1-10293 was used in flow cytometry to clone and study CD93.

Tue Jan 01 00:00:00 EST 2002

Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.

Journal of immunology (Baltimore, Md. : 1950)

Berg LP,James MJ,Alvarez-Iglesias M,Glennie S,Lechler RI,Marelli-Berg FM

MA1-10293 was used in flow cytometry to study the molecular and functional changes induced in T cells that interact with the endothelium.

Mon Apr 01 00:00:00 EST 2002

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

Infection and immunity

McDowell MA,Marovich M,Lira R,Braun M,Sacks D

MA1-10293 was used in flow cytometry to examine the ability of Leishmania species to prime DCs for CD40L-dependent IL-12p70 secretion.

Thu Aug 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86

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Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

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UniProt ID: (Human) P42081

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Entrez Gene ID: (Human) 942

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Function(s)

virus receptor activity receptor activity receptor binding protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

response to yeast adaptive immune response T cell proliferation involved in immune response negative regulation of T cell anergy immune response cell-cell signaling aging positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling T cell costimulation toll-like receptor 3 signaling pathway response to interferon-gamma positive regulation of activated T cell proliferation T cell activation B cell activation response to drug myeloid dendritic cell differentiation positive regulation of lymphotoxin A biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-4 biosynthetic process positive regulation of T-helper 2 cell differentiation positive regulation of transcription, DNA-templated viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus

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CD86 Monoclonal Antibody (BU63)

CD86 Antibody (MA1-10293) in Flow

Product Details

MA1-10293

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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