Invitrogen

CD86 Monoclonal Antibody (BU63), FITC

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

11 References

View all (188) CD86 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (4)
Advanced Verification (1)

FIGURE: 1 / 5

CD86 Antibody (MHCD8601) in Flow

PloS one 2008 - Figure 3 The CaMKKalpha inhibitor STO-609 enhances PMA-dependent up-regulation of CD86 but blocks CD11b expression and CaMKKalpha nuclear localization. (A and B) U937 cells were pretreated with or without 5 ug/ml STO-609 for 6 h prior to activation with 100 nM PMA for 48 h, as indicated. Surface expression of CD11b and CD86 was quantified by flow cytometry using FITC-conjugated CD11b and PE-conjugated CD86 ... View More

Product Image Gallery

CD86 Antibody — Figure 4 Forskolin enhances PMA-dependent CD86 expression while inhibiting CD11b up-regulation and CaMKKalpha nuclear localization. (A and B) U937 cells were pretreated with or without 30 uM forskolin for 1 h prior to activation with 100 nM PMA for 48 h, as indicated. Surface expression of CD11b and CD86 was quantified by flow cytometry using FITC-conjugated CD11b and PE-conjugated CD86 antibodies. Results significantly different from PMA at alpha = 0.05 are indicated by (*). Results represent an average of three independent experiments+-SEM. (C) Cells were treated as in panel A and CaMKKalpha was quantified by Western analysis of nuclear lysates. Results are representative of three independent experiments. (D) U937 cells were treated as in panel A and ERK1/2 phosphorylation (ERK-p) and mass (ERK) were measured by Western analysis in whole cell lysates. Results are representative of three independent experiments. {TM}

Product Details

MHCD8601

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BU63

Immunogen

B-lymphoblastoid cell line ARH 77

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372961

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

MHCD8601 was used in Flow Cytometry to demonstrated that cell cycle-related kinase (CCRK) is upregulated in myeloid cells in tumor-bearing mice and in patients with hepatocellular carcinoma.

Fri Oct 20 00:00:00 EDT 2023

Baicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization.

American journal of cancer research

Zhao X,Qu J,Liu X,Wang J,Ma X,Zhao X,Yang Q,Yan W,Zhao Z,Hui Y,Bai H,Zhang S

MHCD8601 was used in Flow cytometry/Cell sorting to explore the potential effect of baicalein on macrophage polarisation and epithelial-mesenchymal transition of breast cancer.

Wed Sep 30 00:00:00 EDT 2020

Macrophages promote matrix protrusive and invasive function of breast cancer cells via MIP-1β dependent upregulation of MYO3A gene in breast cancer cells.

Oncoimmunology

Baghel KS,Tewari BN,Shrivastava R,Malik SA,Lone MU,Jain NK,Tripathi C,Kanchan RK,Dixit S,Singh K,Mitra K,Negi MP,Srivastava M,Misra S,Bhatt ML,Bhadauria S

MHCD8601 was used in western blot to investigate the role of tumor-associated-macrophages in breast cancer cell invasion and metastasis.

Fri Jul 01 00:00:00 EDT 2016

Induction of type I interferon signaling by Pseudomonas aeruginosa is diminished in cystic fibrosis epithelial cells.

American journal of respiratory cell and molecular biology

Parker D,Cohen TS,Alhede M,Harfenist BS,Martin FJ,Prince A

MHCD8601 was used in Flow cytometry/Cell sorting to suggest that dysfunction of the CFTR in airway epithelial cells may impair immune surveillance in the cystic fibrosis airway.

Sun Jan 01 00:00:00 EST 2012

Ca2+/calmodulin-dependent kinase kinase alpha is expressed by monocytic cells and regulates the activation profile.

PloS one

Guest CB,Deszo EL,Hartman ME,York JM,Kelley KW,Freund GG

Published figure using CD86 monoclonal antibody (Product # MHCD8601) in Flow Cytometry

Wed Feb 13 00:00:00 EST 2008

Ca2+/calmodulin-dependent kinase kinase alpha is expressed by monocytic cells and regulates the activation profile.

PloS one

Guest CB,Deszo EL,Hartman ME,York JM,Kelley KW,Freund GG

Published figure using CD86 monoclonal antibody (Product # MHCD8601) in Flow Cytometry

Wed Feb 13 00:00:00 EST 2008

The immunogenicity of human adipose-derived cells: temporal changes in vitro.

Stem cells (Dayton, Ohio)

McIntosh K,Zvonic S,Garrett S,Mitchell JB,Floyd ZE,Hammill L,Kloster A,Di Halvorsen Y,Ting JP,Storms RW,Goh B,Kilroy G,Wu X,Gimble JM

MHCD8601 was used in Flow cytometry/Cell sorting to compare the immunogenic properties of freshly isolated, collagenase-digested human adipose tissue-derived stromal vascular fraction cells (SVFs) relative to passaged, plastic-adherent adipose-derived stem cells (ASCs).

Mon May 01 00:00:00 EDT 2006

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Journal of virology

Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES

MHCD8601 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.

Tue Jul 01 00:00:00 EDT 2003

CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.

International immunology

Vasu C,Wang A,Gorla SR,Kaithamana S,Prabhakar BS,Holterman MJ

MHCD8601 was used in flow cytometry to elucidate the importance of C domains in the functioning of CD80 and CD86.

Sat Feb 01 00:00:00 EST 2003

CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.

Leukemia

Todisco E,Gaipa G,Biagi E,Bonamino M,Gramigna R,Introna M,Biondi A

MHCD8601 was used in flow cytometry to test if CD40L activates the antigen-presenting cell capacity of B cell precursor acute lymphoblastic leukemia cells.

Tue Oct 01 00:00:00 EDT 2002

Commitment of juvenile myelo-monocytic (JMML) leukemic cells to spontaneously differentiate into dendritic cells.

The hematology journal : the official journal of the European Haematology Association

Longoni D,D'Amico G,Gaipa G,Bernasconi S,Vulcano M,Onnis P,Niemeyer CM,Allavena P,Biondi A

MHCD8601 was used in flow cytometry to study the ability of mononuclear cells from juvenile myelo-monocytic leukemia patients to act as antigen presenting cells.

Fri Sep 26 00:00:00 EDT 2003

Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.

Blood

Ng WF,Duggan PJ,Ponchel F,Matarese G,Lombardi G,Edwards AD,Isaacs JD,Lechler RI

MHCD8601 was used in flow cytometry to characterize CD4(+)CD25(+) human T cells.

Thu Nov 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86

View more View less

Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

View more View less

UniProt ID: (Human) P42081

View more View less

Entrez Gene ID: (Human) 942

View more View less

Function(s)

virus receptor activity receptor activity receptor binding protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

response to yeast adaptive immune response T cell proliferation involved in immune response negative regulation of T cell anergy immune response cell-cell signaling aging positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling T cell costimulation toll-like receptor 3 signaling pathway response to interferon-gamma positive regulation of activated T cell proliferation T cell activation B cell activation response to drug myeloid dendritic cell differentiation positive regulation of lymphotoxin A biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-4 biosynthetic process positive regulation of T-helper 2 cell differentiation positive regulation of transcription, DNA-templated viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD86 Monoclonal Antibody (BU63), FITC

CD86 Antibody (MHCD8601) in Flow

Product Details

MHCD8601

Western Blot (WB)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!