Invitrogen

CD9 Monoclonal Antibody (MM2/57)

FIGURE: 1 / 7

CD9 Antibody (AHS0902) in WB

The EMBO journal 2023 - 3 Figure Involvement of caspase-1 in ILV formation A, B Formation of Tsg101 puncta and co-localization with mtDNA. THP-1 cells transduced with Tsg101-mCherry (red) were stained with PicoGreen (green) and induced to undergo cell death (no death, pyroptosis, or apoptosis). Co-localization of mtDNA with Tsg101 was visualized using confocal microscopy (left). The number of cells expressing Tsg101 puncta (upper)... View More

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Product Details

AHS0902

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

MM2/57

Immunogen

Human platelet glycoprotein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536363

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome.

The EMBO journal

Konaka H,Kato Y,Hirano T,Tsujimoto K,Park J,Koba T,Aoki W,Matsuzaki Y,Taki M,Koyama S,Itotagawa E,Jo T,Hirayama T,Kawai T,Ishii KJ,Ueda M,Yamaguchi S,Akira S,Morita T,Maeda Y,Nishide M,Nishida S,Shima Y,Narazaki M,Takamatsu H,Kumanogoh A

Published figure using CD9 monoclonal antibody (Product # AHS0902) in Western Blot

Mon Oct 16 00:00:00 EDT 2023

Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis.

Cancer science

Liu L,Kawashima M,Sugimoto M,Sonomura K,Pu F,Li W,Takeda M,Goto T,Kawaguchi K,Sato TA,Toi M

Published figure using CD9 monoclonal antibody (Product # AHS0902) in Western Blot

Sun Oct 01 00:00:00 EDT 2023

EVAnalyzer: High content imaging for rigorous characterisation of single extracellular vesicles using standard laboratory equipment and a new open-source ImageJ/Fiji plugin.

Journal of extracellular vesicles

Schürz M,Danmayr J,Jaritsch M,Klinglmayr E,Benirschke HM,Matea CT,Zimmerebner P,Rauter J,Wolf M,Gomes FG,Kratochvil Z,Heger Z,Miller A,Heuser T,Stanojlovic V,Kiefer J,Plank T,Johnson L,Himly M,Blöchl C,Huber CG,Hintersteiner M,Meisner-Kober N

Published figure using CD9 monoclonal antibody (Product # AHS0902) in Western Blot

Thu Dec 01 00:00:00 EST 2022

Next-generation proteomics of serum extracellular vesicles combined with single-cell RNA sequencing identifies MACROH2A1 associated with refractory COVID-19.

Inflammation and regeneration

Kawasaki T,Takeda Y,Edahiro R,Shirai Y,Nogami-Itoh M,Matsuki T,Kida H,Enomoto T,Hara R,Noda Y,Adachi Y,Niitsu T,Amiya S,Yamaguchi Y,Murakami T,Kato Y,Morita T,Yoshimura H,Yamamoto M,Nakatsubo D,Miyake K,Shiroyama T,Hirata H,Adachi J,Okada Y,Kumanogoh A

AHS0902 was used in Western Blotting to highlight that MACROH2A1 in extracellular vesicles is a potential biomarker of refractory COVID-19 and may reflect the pathogenesis of COVID-19 in monocytes.

Wed Nov 30 00:00:00 EST 2022

Next-generation proteomics of serum extracellular vesicles combined with single-cell RNA sequencing identifies MACROH2A1 associated with refractory COVID-19.

Inflammation and regeneration

AHS0902 was used in Western Blotting to highlight that MACROH2A1 in extracellular vesicles is a potential biomarker of refractory COVID-19 and may reflect the pathogenesis of COVID-19 in monocytes.

Wed Nov 30 00:00:00 EST 2022

Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells.

Small (Weinheim an der Bergstrasse, Germany)

Zarubova J,Hasani-Sadrabadi MM,Norris SCP,Majedi FS,Xiao C,Kasko AM,Li S

AHS0902 was used in Western Blotting to develop an in vitro platform based on inverted-pyramidal microwells to follow and quantify the collective migration of hundreds of tumor cell clusters at once.

Thu Dec 01 00:00:00 EST 2022

EZH2 mitigates the cardioprotective effects of mesenchymal stem cell-secreted exosomes against infarction via HMGA2-mediated PI3K/AKT signaling.

BMC cardiovascular disorders

Jiao W,Hao J,Xie Y,Meng M,Gao W

AHS0902 was used in Western Blot to show that MSC-EXO alleviated fbrosis in MI rats via inhibition of EZH2, whereas EZH2 inhibited HMGA2 expression and impaired the PI3K/AKT pathway.

Wed Mar 09 00:00:00 EST 2022

Isolation of Small Extracellular Vesicles from Human Sera.

International journal of molecular sciences

Małys MSS,Aigner C,Schulz SMM,Schachner H,Rees AJJ,Kain R

Published figure using CD9 monoclonal antibody (Product # AHS0902) in Western Blot

Wed Apr 28 00:00:00 EDT 2021

Proteomic Analysis of Circulating Extracellular Vesicles Identifies Potential Biomarkers for Lymph Node Metastasis in Oral Tongue Squamous Cell Carcinoma.

Cells

Qu X,Leung TCN,Ngai SM,Tsai SN,Thakur A,Li WK,Lee Y,Leung L,Ng TH,Yam J,Lan L,Lau EHL,Wong EWY,Chan JYK,Meehan K

AHS0902 was used in Western Blot to conclude that the shortlisted EV associated proteins described here may be useful discriminatory biomarkers for differentiating OTSCC with and without nodal disease or non-cancer controls.

Tue Aug 24 00:00:00 EDT 2021

Extracellular vesicles in follicular fluid of sexually mature gilts' ovarian antral follicles - identification and proteomic analysis.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society

Grzesiak M,Popiolek K,Knapczyk-Stwora K

AHS0902 was used in Western Blotting to shows the presence of EVs in follicular fluid of small (SFs), medium (MFs) and large (LFs) antral follicles of sexually mature gilts using nanoparticle tracking analysis and Western blot.

Sat Feb 01 00:00:00 EST 2020

Self-Replicating RNA Vaccine Delivery to Dendritic Cells.

Methods in molecular biology (Clifton, N.J.)

Démoulins T,Englezou PC,Milona P,Ruggli N,Tirelli N,Pichon C,Sapet C,Ebensen T,Guzmán CA,McCullough KC

AHS0902 was used in immunocytochemistry to use biodegradable vaccine delivery vehicles to protect self-amplifying replicon RNA and promote delivery to dendritic cells

Wed Jan 10 00:00:00 EST 2018

CD151-α3β1 integrin complexes are prognostic markers of glioblastoma and cooperate with EGFR to drive tumor cell motility and invasion.

Oncotarget

Zhou P,Erfani S,Liu Z,Jia C,Chen Y,Xu B,Deng X,Alfáro JE,Chen L,Napier D,Lu M,Huang JA,Liu C,Thibault O,Segal R,Zhou BP,Kyprianou N,Horbinski C,Yang XH

AHS0902 was used in flow cytometry to elucidate the role of CD151-alpha3beta1 integrin complexes in its cooperation with EGFR to drive tumor cell motility and invasion and as prognositic markers of glioblastoma

Tue Oct 06 00:00:00 EDT 2015

Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.

PloS one

Jin Y,Tachibana I,Takeda Y,He P,Kang S,Suzuki M,Kuhara H,Tetsumoto S,Tsujino K,Minami T,Iwasaki T,Nakanishi K,Kohmo S,Hirata H,Takahashi R,Inoue K,Nagatomo I,Kida H,Kijima T,Ito M,Saya H,Kumanogoh A

AHS0902 was used in western blot to identify agents that increase the levels of tetraspanins in macrophages.

Wed Jul 02 00:00:00 EDT 2014

Cell surface tetraspanin CD9 mediates chemoresistance in small cell lung cancer.

Cancer research

Kohmo S,Kijima T,Otani Y,Mori M,Minami T,Takahashi R,Nagatomo I,Takeda Y,Kida H,Goya S,Yoshida M,Kumagai T,Tachibana I,Yokota S,Kawase I

Fri Oct 15 00:00:00 EDT 2010

Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells.

European journal of cell biology

Castro-Sanchez L,Soto-Guzman A,Navarro-Tito N,Martinez-Orozco R,Salazar EP

AHS0902 was used in western blot to elucidate the contribution of DDR1 and type IV collagen to regulation of CD9-cell surface levels and migration of breast cancer cells

Mon Nov 01 00:00:00 EDT 2010

Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis.

Molecular biology of the cell

Lafleur MA,Xu D,Hemler ME

AHS0902 was used in immunocytochemistry and western blot to investigate the role of MT1-MMP-tetraspanin complexes in cancer cell invasion and metastasis

Wed Apr 01 00:00:00 EDT 2009

CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization.

Cancer research

Yang XH,Richardson AL,Torres-Arzayus MI,Zhou P,Sharma C,Kazarov AR,Andzelm MM,Strominger JL,Brown M,Hemler ME

AHS0902 was used in western blot to determine the contribution of CD151 on breast cancer

Thu May 01 00:00:00 EDT 2008

Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.

The Journal of biological chemistry

Takeda Y,He P,Tachibana I,Zhou B,Miyado K,Kaneko H,Suzuki M,Minami S,Iwasaki T,Goya S,Kijima T,Kumagai T,Yoshida M,Osaki T,Komori T,Mekada E,Kawase I

AHS0902 was used in western blot to examine the roles of CD9 and CD81 in cell motility and protease production of smoke-treated macrophages

Fri Sep 19 00:00:00 EDT 2008

Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells.

Cancer research

Saito Y,Tachibana I,Takeda Y,Yamane H,He P,Suzuki M,Minami S,Kijima T,Yoshida M,Kumagai T,Osaki T,Kawase I

AHS0902 was used in flow cytometry, immunoprecipitation, and western blot to investigate the role of CD9 in small cell lung cancer cells

Sun Oct 01 00:00:00 EDT 2006

CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen.

Thrombosis and haemostasis

Israels SJ,McMillan-Ward EM

Tue Feb 01 00:00:00 EST 2005

CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen.

Thrombosis and haemostasis

Israels SJ,McMillan-Ward EM

Tue Feb 01 00:00:00 EST 2005

cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.

The Journal of biological chemistry

Lanza F,Wolf D,Fox CF,Kieffer N,Seyer JM,Fried VA,Coughlin SR,Phillips DR,Jennings LK

AHS0902 was used in immunoprecipitation to clone, sequence, and express the full-length cDNA for the human platelet p24/CD9 antigen.

Wed Jun 05 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

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Gene Aliases: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

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UniProt ID: (Human) P21926, (Mouse) P40240

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Entrez Gene ID: (Rabbit) 100345844, (Human) 928, (Mouse) 12527

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Function(s)

integrin binding protein binding

Process(es)

movement of cell or subcellular component cell adhesion single fertilization fusion of sperm to egg plasma membrane negative regulation of cell proliferation paranodal junction assembly platelet degranulation cell surface receptor signaling pathway brain development response to water deprivation oligodendrocyte development platelet activation receptor internalization cellular response to low-density lipoprotein particle stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD9 Monoclonal Antibody (MM2/57)

CD9 Antibody (AHS0902) in WB

Product Details

AHS0902

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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