Invitrogen

CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (7)

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CD9 Antibody (12-0098-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD9 PE (purple histogram). Cells in the monocyte gate were used for analysis.

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Product Details

12-0098-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSN4 (SN4 C3-3A2)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854122

Product Specific Information

Description: The eBioSN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the eBioSN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

Applications Reported: This eBioSN4 (SN4 C3-3A2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN4 (SN4 C3-3A2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells.

Cells

Santos MF,Rappa G,Fontana S,Karbanová J,Aalam F,Tai D,Li Z,Pucci M,Alessandro R,Morimoto C,Corbeil D,Lorico A

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Wed Aug 10 00:00:00 EDT 2022

Extracellular Vesicle Membrane Protein Profiling and Targeted Mass Spectrometry Unveil CD59 and Tetraspanin 9 as Novel Plasma Biomarkers for Detection of Colorectal Cancer.

Cancers

Dash S,Wu CC,Wu CC,Chiang SF,Lu YT,Yeh CY,You JF,Chu LJ,Yeh TS,Yu JS

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Wed Dec 28 00:00:00 EST 2022

Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex.

Cell reports

Mich JK,Graybuck LT,Hess EE,Mahoney JT,Kojima Y,Ding Y,Somasundaram S,Miller JA,Kalmbach BE,Radaelli C,Gore BB,Weed N,Omstead V,Bishaw Y,Shapovalova NV,Martinez RA,Fong O,Yao S,Mortrud M,Chong P,Loftus L,Bertagnolli D,Goldy J,Casper T,Dee N,Opitz-Araya X,Cetin A,Smith KA,Gwinn RP,Cobbs C,Ko AL,Ojemann JG,Keene CD,Silbergeld DL,Sunkin SM,Gradinaru V,Horwitz GD,Zeng H,Tasic B,Lein ES,Ting JT,Levi BP

12-0098-42 was used in Flow cytometry/Cell sorting to demonstrate how genome-wide open chromatin data mining and cross-species AAV validation can be used to create the next generation of non-species-restricted viral genetic tools.

Tue Mar 30 00:00:00 EDT 2021

CD9 knockdown suppresses cell proliferation, adhesion, migration and invasion, while promoting apoptosis and the efficacy of chemotherapeutic drugs and imatinib in Ph+ ALL SUP‑B15 cells.

Molecular medicine reports

Xing C,Xu W,Shi Y,Zhou B,Wu D,Liang B,Zhou Y,Gao S,Feng J

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CD9 knockdown suppresses cell proliferation, adhesion, migration and invasion, while promoting apoptosis and the efficacy of chemotherapeutic drugs and imatinib in Ph+ ALL SUP‑B15 cells.

Molecular medicine reports

Xing C,Xu W,Shi Y,Zhou B,Wu D,Liang B,Zhou Y,Gao S,Feng J

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

MLL leukemia induction by t(9;11) chromosomal translocation in human hematopoietic stem cells using genome editing.

Blood advances

Schneidawind C,Jeong J,Schneidawind D,Kim IS,Duque-Afonso J,Wong SHK,Iwasaki M,Breese EH,Zehnder JL,Porteus M,Cleary ML

12-0098 was used in Flow cytometry/Cell sorting to study the use of genome editing for the modelling of human acute MLL-rearranged leukaemia in vivo.

Tue Apr 24 00:00:00 EDT 2018

Eomesodermin and T-bet mark developmentally distinct human natural killer cells.

JCI insight

Collins A,Rothman N,Liu K,Reiner SL

12-0098 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation.

Thu Mar 09 00:00:00 EST 2017

Human islets contain four distinct subtypes of β cells.

Nature communications

Dorrell C,Schug J,Canaday PS,Russ HA,Tarlow BD,Grompe MT,Horton T,Hebrok M,Streeter PR,Kaestner KH,Grompe M

Published figure using CD9 monoclonal antibody (Product # 12-0098-42) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types.

PloS one

Momose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H

12-0098 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich sequences are a dominant feature of exosome-dominant miRNAs.

Wed Apr 05 00:00:00 EDT 2017

A pilot study of omalizumab in eosinophilic esophagitis.

PloS one

Loizou D,Enav B,Komlodi-Pasztor E,Hider P,Kim-Chang J,Noonan L,Taber T,Kaushal S,Limgala R,Brown M,Gupta R,Balba N,Goker-Alpan O,Khojah A,Alpan O

12-0098 was used in Flow cytometry/Cell sorting to demonstrate that anti-IgE therapy with omalizumab may result in Eosinophilic Esophagitis disease remission.

Thu Feb 04 00:00:00 EST 2016

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

12-0098 was used in Flow cytometry/Cell sorting to investigate microvesicles derived from human umbilical cord mesenchymal stem cells, and their ability to attenuate bladder tumour cell growth.

Tue Oct 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

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Gene Aliases: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

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UniProt ID: (Human) P21926

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Entrez Gene ID: (Human) 928

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Function(s)

integrin binding protein binding

Process(es)

platelet degranulation movement of cell or subcellular component cell adhesion cell surface receptor signaling pathway fusion of sperm to egg plasma membrane brain development negative regulation of cell proliferation response to water deprivation oligodendrocyte development platelet activation paranodal junction assembly receptor internalization cellular response to low-density lipoprotein particle stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), PE, eBioscience™

CD9 Antibody (12-0098-42) in Flow

Product Details

12-0098-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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