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Invitrogen
CD90 Monoclonal Antibody (F15-42-1)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
CD90 Antibody (MA5-16671) in ICC/IF
Immunofluorescence analysis of CD90 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More
Product Details
MA5-16671
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F15-42-1
Immunogen
Purified human brain Thy-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538165
Product Specific Information
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human brain or thymus. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.
Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.
Target Information
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen
Gene Aliases: CD90; CDw90; THY1
UniProt ID: (Human) P04216
Entrez Gene ID: (Human) 7070, (Cynomolgus monkey) 102139037
angiogenesis
negative regulation of protein kinase activity
cytoskeleton organization
single organismal cell-cell adhesion
negative regulation of cell migration
positive regulation of GTPase activity
retinal cone cell development
protein autophosphorylation
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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