Invitrogen

CD90 (Thy-1) Monoclonal Antibody (YKIX337.217), APC, eBioscience™

CD90 (Thy-1) Antibody in Flow Cytometry (Flow)

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CD90 (Thy-1) Antibody (17-5900-42) in Flow

Staining of normal canine peripheral blood cells with Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (blue histogram) or Anti-Canine CD90 (Thy-1) APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-5900-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

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Product Specifications

Species Reactivity

Dog

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YKIX337.217

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717512

Product Specific Information

Description: This YKIX337.217 monoclonal antibody reacts with canine CD90, which is also known as Thy-1. This 25-37 kDa N-glycoylsated cell adhesion molecule is expressed on thymocytes, peripheral T cells, some B cells, monocytes, and granulocytes albeit at a lower level. CD90 is also expressed on cells in the bone marrow, brain, and kidney, as well as on mesenchymal stem cells.

This monoclonal antibody has been reported to deplete cells in vitro and in vivo.

Applications Reported: This YKIX337.217 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX337.217 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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