Invitrogen

CD93 (AA4.1) Monoclonal Antibody (AA4.1), Biotin, eBioscience™

5 Published Figures

17 References

View all (29) CD93 (AA4.1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

CD93 (AA4.1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD93 (AA4.1) Antibody (13-5892-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (left) or 0.125 µg of Anti-Mouse CD93 (AA4.1) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5892-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AA4.1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466767

Product Specific Information

Description: The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1^+ cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1^+/CD23^-/sIgMhi), T2 (AA4.1^+/CD23^+/sIgMhi), and T3 (AA4.1^+/CD23^+/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported: The AA4.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 13-5892-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Clonal lineage tracing reveals shared origin of conventional and plasmacytoid dendritic cells.

Immunity

Feng J,Pucella JN,Jang G,Alcántara-Hernández M,Upadhaya S,Adams NM,Khodadadi-Jamayran A,Lau CM,Stoeckius M,Hao S,Smibert P,Tsirigos A,Idoyaga J,Reizis B

13-5892-82 was used in Flow cytometry/Cell sorting to reveal the shared origin of pDCs and cDCs and suggest a revised scheme of DC development whereby pDCs share clonal relationship with cDC1s.

Tue Mar 08 00:00:00 EST 2022

Hyper-metabolic B cells in the spleens of old mice make antibodies with autoimmune specificities.

Immunity & ageing : I & A

Frasca D,Romero M,Garcia D,Diaz A,Blomberg BB

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 13-5892-82) in Flow Cytometry

Sat Feb 27 00:00:00 EST 2021

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

13-5892-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 EDT 2020

Activated PI3Kδ breaches multiple B cell tolerance checkpoints and causes autoantibody production.

The Journal of experimental medicine

Lau A,Avery DT,Jackson K,Lenthall H,Volpi S,Brigden H,Russell AJ,Bier J,Reed JH,Smart JM,Cole T,Choo S,Gray PE,Berglund LJ,Hsu P,Wong M,O'Sullivan M,Boztug K,Meyts I,Uzel G,Notarangelo LD,Brink R,Goodnow CC,Tangye SG,Deenick EK

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 13-5892-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes.

The Journal of experimental medicine

Bancroft T,DeBuysscher BL,Weidle C,Schwartz A,Wall A,Gray MD,Feng J,Steach HR,Fitzpatrick KS,Gewe MM,Skog PD,Doyle-Cooper C,Ota T,Strong RK,Nemazee D,Pancera M,Stamatatos L,McGuire AT,Taylor JJ

13-5892-82 was used in Functional assay to indicate that anti-idiotypes are a valuable tool for the study and induction of potentially protective antibodies.

Mon Oct 07 00:00:00 EDT 2019

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 13-5892-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

The microRNA miR-148a functions as a critical regulator of B cell tolerance and autoimmunity.

Nature immunology

Gonzalez-Martin A,Adams BD,Lai M,Shepherd J,Salvador-Bernaldez M,Salvador JM,Lu J,Nemazee D,Xiao C

13-5892 was used in Magnetic cell separation to demonstrate a function for miR-148a as a regulator of B cell tolerance and autoimmunity.

Fri Apr 01 00:00:00 EDT 2016

IgD attenuates the IgM-induced anergy response in transitional and mature B cells.

Nature communications

Sabouri Z,Perotti S,Spierings E,Humburg P,Yabas M,Bergmann H,Horikawa K,Roots C,Lambe S,Young C,Andrews TD,Field M,Enders A,Reed JH,Goodnow CC

13-5892 was used in Flow cytometry/Cell sorting to define the RNA landscape of the in vivo anergy response, comprising 220 induced sequences including a core set of 97.

Thu Nov 10 00:00:00 EST 2016

ATP11C Facilitates Phospholipid Translocation across the Plasma Membrane of All Leukocytes.

PloS one

Yabas M,Jing W,Shafik S,Bröer S,Enders A

13-5892 was used in Flow cytometry/Cell sorting to identify the P4-type ATPase ATP11C as a flippase that selectively transports phosphatidylserine and phosphatidylethanolamine to the inner leaflet of the plasma membrane in immune cells.

Mon Jul 25 00:00:00 EDT 2016

CD93 Marks a Non-Quiescent Human Leukemia Stem Cell Population and Is Required for Development of MLL-Rearranged Acute Myeloid Leukemia.

Cell stem cell

Iwasaki M,Liedtke M,Gentles AJ,Cleary ML

13-5892 was used in Flow cytometry/Cell sorting to study how CD93 expression may indicate leukaemia initiating stem cells that have a MLL gene rearrangement.

Thu Oct 01 00:00:00 EDT 2015

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

13-5892 was used in Flow cytometry/Cell sorting to conclude that chronic inflammatory microenvironments in old age lead to reductions in the fitness of B cell progenitor populations.

Tue Dec 01 00:00:00 EST 2015

Distinct germinal center selection at local sites shapes memory B cell response to viral escape.

The Journal of experimental medicine

Adachi Y,Onodera T,Yamada Y,Daio R,Tsuiji M,Inoue T,Kobayashi K,Kurosaki T,Ato M,Takahashi Y

13-5892 was used in Flow cytometry/Cell sorting to investigate the cellular events dictating the cross-reactive memory B cell responses, showing that distinct germinal center selection at local sites shapes response to viral escape.

Mon Sep 21 00:00:00 EDT 2015

Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.

The Journal of experimental medicine

Kaji T,Ishige A,Hikida M,Taka J,Hijikata A,Kubo M,Nagashima T,Takahashi Y,Kurosaki T,Okada M,Ohara O,Rajewsky K,Takemori T

13-5892 was used in Flow cytometry/Cell sorting to elucidate two differentiation pathways involved in the generation of B cell memory from germline-encoded and somatically mutated antibodies.

Mon Oct 22 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 13-5892-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

13-5892 was used in Magnetic cell separation to demonstrate that nuclear export of IκBα is essential in maintaining constitutive, canonical, and noncanonical NF-κB activation potentials in mature B cells in vivo.

Fri Feb 25 00:00:00 EST 2011

Irradiation selects for p53-deficient hematopoietic progenitors.

PLoS biology

Marusyk A,Porter CC,Zaberezhnyy V,DeGregori J

13-5892 was used in Flow cytometry/Cell sorting to elucidate the mechanism behind the carcinogenic effect of ionising radiation.

Tue Mar 02 00:00:00 EST 2010

Bioinformatics

Protein Aliases: C1q receptor; C1q/MBL/SPA receptor; C1qR(p); CD93; Cell surface antigen AA4; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; Complement component C1q receptor; Ly-68; Lymphocyte antigen 68

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Gene Aliases: 6030404G09Rik; AA145088; Aa4; AA4.1; AW555904; C1qr1; C1qrp; Cd93; Ly68

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UniProt ID: (Mouse) O89103

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Entrez Gene ID: (Mouse) 17064

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Function(s)

complement component C1q binding calcium ion binding carbohydrate binding extracellular matrix structural protein

Process(es)

positive regulation of defense response to virus by host cell adhesion single organismal cell-cell adhesion mitophagy in response to mitochondrial depolarization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD93 (AA4.1) Monoclonal Antibody (AA4.1), Biotin, eBioscience™

CD93 (AA4.1) Antibody (13-5892-82) in Flow

Product Details

13-5892-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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