Invitrogen

CD93 (AA4.1) Monoclonal Antibody (AA4.1), PE-Cyanine7, eBioscience™

CD93 (AA4.1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD93 (AA4.1) Antibody (25-5892-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.125 µg of CD93 (AA4-1) PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-5892-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AA4.1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469659

Product Specific Information

Description: The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1 positive cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1+/CD23-/sIgMhi), T2 (AA4.1+/CD23+/sIgMhi), and T3 (AA4.1+/CD23+/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported: This AA4.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Hyper-metabolic B cells in the spleens of old mice make antibodies with autoimmune specificities.

Immunity & ageing : I & A

Frasca D,Romero M,Garcia D,Diaz A,Blomberg BB

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Sat Feb 27 00:00:00 EST 2021

Hyper-metabolic B cells in the spleens of old mice make antibodies with autoimmune specificities.

Immunity & ageing : I & A

Frasca D,Romero M,Garcia D,Diaz A,Blomberg BB

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Sat Feb 27 00:00:00 EST 2021

Neonatal Exposure to Commensal-Bacteria-Derived Antigens Directs Polysaccharide-Specific B-1 B Cell Repertoire Development.

Immunity

New JS,Dizon BLP,Fucile CF,Rosenberg AF,Kearney JF,King RG

25-5892-82 was used in Flow Cytometry to show that exposure to microbial antigens in early life determines the clonality of the mature B-1 B cell repertoire and ensuing antibody responses, with implications for vaccination approaches and schedules.

Tue Jul 14 00:00:00 EDT 2020

Activated PI3Kδ breaches multiple B cell tolerance checkpoints and causes autoantibody production.

The Journal of experimental medicine

Lau A,Avery DT,Jackson K,Lenthall H,Volpi S,Brigden H,Russell AJ,Bier J,Reed JH,Smart JM,Cole T,Choo S,Gray PE,Berglund LJ,Hsu P,Wong M,O'Sullivan M,Boztug K,Meyts I,Uzel G,Notarangelo LD,Brink R,Goodnow CC,Tangye SG,Deenick EK

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Obesity induces pro-inflammatory B cells and impairs B cell function in old mice.

Mechanisms of ageing and development

Frasca D,Diaz A,Romero M,Vazquez T,Blomberg BB

25-5892 was used in Flow cytometry/Cell sorting to show a direct effect of adipocytes on the generation of pro-inflammatory B cells.

Wed Mar 01 00:00:00 EST 2017

IL-1-induced Bhlhe40 identifies pathogenic T helper cells in a model of autoimmune neuroinflammation.

The Journal of experimental medicine

Lin CC,Bradstreet TR,Schwarzkopf EA,Jarjour NN,Chou C,Archambault AS,Sim J,Zinselmeyer BH,Carrero JA,Wu GF,Taneja R,Artyomov MN,Russell JH,Edelson BT

25-5892 was used in Flow cytometry/Cell sorting to demonstrate that IL-1β induces Bhlhe40 expression in polarized Th17 cells, and Bhlhe40-expressing cells exhibited an encephalitogenic transcriptional signature.

Mon Feb 08 00:00:00 EST 2016

CD93 Marks a Non-Quiescent Human Leukemia Stem Cell Population and Is Required for Development of MLL-Rearranged Acute Myeloid Leukemia.

Cell stem cell

Iwasaki M,Liedtke M,Gentles AJ,Cleary ML

25-5892 was used in Flow cytometry/Cell sorting to study how CD93 expression may indicate leukaemia initiating stem cells that have a MLL gene rearrangement.

Thu Oct 01 00:00:00 EDT 2015

VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection.

Journal of immunology (Baltimore, Md. : 1950)

Palmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC

25-5892 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection.

Sat Aug 15 00:00:00 EDT 2015

Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires.

eLife

Yang Y,Wang C,Yang Q,Kantor AB,Chu H,Ghosn EE,Qin G,Mazmanian SK,Han J,Herzenberg LA

25-5892 was used in Flow cytometry/Cell sorting to show that V(D)J selection and AID-mediated diversification operate comparably in germ-free and conventional mice.

Wed Sep 30 00:00:00 EDT 2015

IL-4 regulates Bim expression and promotes B cell maturation in synergy with BAFF conferring resistance to cell death at negative selection checkpoints.

Journal of immunology (Baltimore, Md. : 1950)

Granato A,Hayashi EA,Baptista BJ,Bellio M,Nobrega A

Sun Jun 15 00:00:00 EDT 2014

In senescence, age-associated B cells secrete TNFα and inhibit survival of B-cell precursors.

Aging cell

Ratliff M,Alter S,Frasca D,Blomberg BB,Riley RL

25-5892 was used in Flow cytometry/Cell sorting to elucidate the differential cytokine expression by old and young follicular splenic (FO) B cells, and the effects on age-associated B cells (ABCs).

Mon Apr 01 00:00:00 EDT 2013

In vivo knockdown of TAK1 accelerates bone marrow proliferation/differentiation and induces systemic inflammation.

PloS one

Vink PM,Smout WM,Driessen-Engels LJ,de Bruin AM,Delsing D,Krajnc-Franken MA,Jansen AJ,Rovers EF,van Puijenbroek AA,Kaptein A,Nolte MA,Garritsen A,van Eenennaam H

25-5892 was used in Flow cytometry/Cell sorting to study the function of TAK1 protein in the adult immune system.

Fri Sep 06 00:00:00 EDT 2013

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

25-5892-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD93 (AA4.1) monoclonal antibody (Product # 25-5892-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

TLR4 promotes B cell maturation: independence and cooperation with B lymphocyte-activating factor.

Journal of immunology (Baltimore, Md. : 1950)

Hayashi EA,Granato A,Paiva LS,Bertho AL,Bellio M,Nobrega A

25-5892 was used in Flow cytometry/Cell sorting to perform an extensive study of cell surface markers and functional properties of B cells matured in vitro with LPS.

Sat May 01 00:00:00 EDT 2010

Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.

Journal of immunology (Baltimore, Md. : 1950)

Duong BH,Ota T,Aït-Azzouzene D,Aoki-Ota M,Vela JL,Huber C,Walsh K,Gavin AL,Nemazee D

25-5892 was used in Flow cytometry/Cell sorting to generate mice which ubiquitously expressed a membrane-bound IgD-superantigen, to provide a model which should facilitate studies of peripheral B cell tolerance in future.

Thu Apr 15 00:00:00 EDT 2010

HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion.

PloS one

Königsberger S,Peckl-Schmid D,Zaborsky N,Patzak I,Kiefer F,Achatz G

25-5892 was used in Flow cytometry/Cell sorting to conclude that HPK1 exhibits a dual function in B-cells by negatively regulating integrin activity and controlling cellular activation.

Wed Sep 01 00:00:00 EDT 2010

Antibodies in a heavy chain knock-in mouse exhibit characteristics of early heavy chain rearrangement.

Journal of immunology (Baltimore, Md. : 1950)

Yunk L,Meng W,Cohen PL,Eisenberg RA,Luning Prak ET

25-5892 was used in Flow cytometry/Cell sorting to investigate the antibody heavy chain rearrangement that preceeds B cell receptor expression.

Wed Jul 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C1q receptor; C1q/MBL/SPA receptor; C1qR(p); CD93; Cell surface antigen AA4; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; Complement component C1q receptor; Ly-68; Lymphocyte antigen 68

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Gene Aliases: 6030404G09Rik; AA145088; Aa4; AA4.1; AW555904; C1qr1; C1qrp; Cd93; Ly68

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UniProt ID: (Mouse) O89103

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Entrez Gene ID: (Mouse) 17064

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Function(s)

complement component C1q binding calcium ion binding carbohydrate binding extracellular matrix structural protein

Process(es)

positive regulation of defense response to virus by host cell adhesion single organismal cell-cell adhesion mitophagy in response to mitochondrial depolarization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD93 (AA4.1) Monoclonal Antibody (AA4.1), PE-Cyanine7, eBioscience™

CD93 (AA4.1) Antibody (25-5892-82) in Flow

Product Details

25-5892-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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