Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD95 (APO-1/Fas) Monoclonal Antibody (APO-1-1), FITC, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CD95 (APO-1/Fas) Antibody (BMS151FI) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 PE-Cy7 and CD95 FITC. As expected based on known relative expression patterns, CD95 clone APO-1-1 stains a subset of lymphocytes (pink), monocytes (orange) and not granulocytes (blue).
Product Details
BMS151FI
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
APO-1-1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10596648
Product Specific Information
Description: Immunohistochemistry:
APO-1-1 (BMS151, IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, BMS151 is especially useful for immunohistology on cryostat sections and on cytospin preparations.
Flow cytometry:
BMS151 is also suitable as primary antibody in staining for FACS analysis, BMS151FI can be used for direct staining of cells, BMS151BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended concentration for the unlabelled antibody is 1 - 2 µg/mL and a 1:20 dilution of the labelled antibodies, as determined on SKW6.4 cells.
Applications Tested: Flow Cytometry.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Target Information
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6
UniProt ID: (Human) P25445
Entrez Gene ID: (Human) 355
ovarian follicle atresia
immunoglobulin production
renal system process
protein complex assembly
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
activation-induced cell death of T cells
inflammatory cell apoptotic process
inflammatory response
immune response
signal transduction
spermatogenesis
brain development
aging
circadian rhythm
extrinsic apoptotic signaling pathway via death domain receptors
response to toxic substance
gene expression
regulation of gene expression
B cell mediated immunity
telencephalon development
dendrite regeneration
positive regulation of protein homooligomerization
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
response to drug
ovulation cycle
regulation of apoptotic process
chordate embryonic development
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
response to peptide hormone
negative thymic T cell selection
regulation of lymphocyte differentiation
regulation of myeloid cell differentiation
response to cycloheximide
spleen development
negative regulation of B cell activation
protein homooligomerization
response to glucocorticoid
maternal process involved in female pregnancy
positive regulation of lymphocyte apoptotic process
cellular response to hydrogen peroxide
response to growth factor
cellular response to phenylalanine
cellular response to mechanical stimulus
cellular response to cobalt ion
cellular response to lithium ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to estrogen stimulus
cellular response to hyperoxia
cellular response to hypoxia
cellular response to hydrostatic pressure
motor neuron apoptotic process
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
hepatocyte apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
liver regeneration
necroptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
response to fluoride
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support