Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (DX2), PE-Cyanine5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

8 Published Figures

15 References

View all (107) FAS (CD95) antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD95 (APO-1/Fas) Antibody (15-0959-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 FITC and CD95 PE-Cyanine5 or isotype control mouse IgG1 PE-Cyanine5. As expected based on known relative expression patterns, CD95 clone DX2 stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink).

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Product Details

15-0959-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Dog, Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DX2

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042290

Product Specific Information

Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

The DX2 monoclonal is reported to recognize dog/canine CD95.

Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.

Nature immunology

Xu Q,Milanez-Almeida P,Martins AJ,Radtke AJ,Hoehn KB,Oguz C,Chen J,Liu C,Tang J,Grubbs G,Stein S,Ramelli S,Kabat J,Behzadpour H,Karkanitsa M,Spathies J,Kalish H,Kardava L,Kirby M,Cheung F,Preite S,Duncker PC,Kitakule MM,Romero N,Preciado D,Gitman L,Koroleva G,Smith G,Shaffer A,McBain IT,McGuire PJ,Pittaluga S,Germain RN,Apps R,Schwartz DM,Sadtler K,Moir S,Chertow DS,Kleinstein SH,Khurana S,Tsang JS,Mudd P,Schwartzberg PL,Manthiram K

15-0959-42 was used in Flow cytometry/Cell sorting to provide evidence for persistent tissue-specific immunity to SARS-CoV-2 in the upper respiratory tract of children after infection.

Sun Jan 01 00:00:00 EST 2023

Vaccine plus microbicide effective in preventing vaginal SIV transmission in macaques.

Nature microbiology

Rahman MA,Bissa M,Silva de Castro I,Helmold Hait S,Stamos JD,Bhuyan F,Hunegnaw R,Sarkis S,Gutowska A,Doster MN,Moles R,Hoang T,Miller Jenkins LM,Appella E,Venzon DJ,Choo-Wosoba H,Cardozo T,Baum MM,Appella DH,Robert-Guroff M,Franchini G

15-0959-42 was used in Flow cytometry/Cell sorting to show that, by increasing zinc availability, SAMT-247 increases natural killer cytotoxicity and monocyte efferocytosis, and decreases T-cell activation to augment vaccine-induced protection.

Mon May 01 00:00:00 EDT 2023

Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.

Immunity

Kreutmair S,Unger S,Núñez NG,Ingelfinger F,Alberti C,De Feo D,Krishnarajah S,Kauffmann M,Friebel E,Babaei S,Gaborit B,Lutz M,Jurado NP,Malek NP,Goepel S,Rosenberger P,Häberle HA,Ayoub I,Al-Hajj S,Nilsson J,Claassen M,Liblau R,Martin-Blondel G,Bitzer M,Roquilly A,Becher B

15-0959-42 was used in Flow Cytometry to identify pathological immune signatures suggestive of T cell exhaustion unique to COVID-19 infection.

Tue Jul 13 00:00:00 EDT 2021

Mucosal-associated invariant T cell responses differ by sex in COVID-19.

Med (New York, N.Y.)

Yu C,Littleton S,Giroux NS,Mathew R,Ding S,Kalnitsky J,Yang Y,Petzold E,Chung HA,Rivera GO,Rotstein T,Xi R,Ko ER,Tsalik EL,Sempowski GD,Denny TN,Burke TW,McClain MT,Woods CW,Shen X,Saban DR

15-0959-42 was used in Flow Cytometry to reveal a pronounced reduction of circulating mucosal-associated invariant T (MAIT) cells in infected females.

Fri Jun 11 00:00:00 EDT 2021

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Simian Immunodeficiency Virus Infection of Rhesus Macaques Results in Delayed Zika Virus Clearance.

mBio

Vinton CL,Magaziner SJ,Dowd KA,Robertson SJ,Amaro-Carambot E,Karmele EP,Ortiz AM,Starke CE,Mudd JC,Whitehead SS,Best SM,Pierson TC,Hickman HD,Brenchley JM

15-0959-42 was used in Flow Cytometry to highlight how individual components of the innate and adaptive immune responses which become perturbed in HIV-infected individuals influence ZIKV infection.

Tue Dec 03 00:00:00 EST 2019

Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Mou H,Moore J,Malonia SK,Li Y,Ozata DM,Hough S,Song CQ,Smith JL,Fischer A,Weng Z,Green MR,Xue W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Tue Apr 04 00:00:00 EDT 2017

Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Mou H,Moore J,Malonia SK,Li Y,Ozata DM,Hough S,Song CQ,Smith JL,Fischer A,Weng Z,Green MR,Xue W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Tue Apr 04 00:00:00 EDT 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.

Oncotarget

Zhang M,Wang J,Jia L,Huang J,He C,Hu F,Yuan L,Wang G,Yu M,Li Z

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Fri Sep 08 00:00:00 EDT 2017

Mucosal B Cells Are Associated with Delayed SIV Acquisition in Vaccinated Female but Not Male Rhesus Macaques Following SIVmac251 Rectal Challenge.

PLoS pathogens

Tuero I,Mohanram V,Musich T,Miller L,Vargas-Inchaustegui DA,Demberg T,Venzon D,Kalisz I,Kalyanaraman VS,Pal R,Ferrari MG,LaBranche C,Montefiori DC,Rao M,Vaccari M,Franchini G,Barnett SW,Robert-Guroff M

15-0959 was used in Flow cytometry/Cell sorting to study the cellular immune response in an intracellular cytokine assay.

Sat Aug 01 00:00:00 EDT 2015

Target cell availability, rather than breast milk factors, dictates mother-to-infant transmission of SIV in sooty mangabeys and rhesus macaques.

PLoS pathogens

Chahroudi A,Cartwright E,Lee ST,Mavigner M,Carnathan DG,Lawson B,Carnathan PM,Hashempoor T,Murphy MK,Meeker T,Ehnert S,Souder C,Else JG,Cohen J,Collman RG,Vanderford TH,Permar SR,Derdeyn CA,Villinger F,Silvestri G

15-0959 was used in Flow cytometry/Cell sorting to elucidate the factors that prevent the vertical transmission of SIV in sooty mangabeys.

Sat Mar 01 00:00:00 EST 2014

Target cell availability, rather than breast milk factors, dictates mother-to-infant transmission of SIV in sooty mangabeys and rhesus macaques.

PLoS pathogens

15-0959 was used in Flow cytometry/Cell sorting to elucidate the factors that prevent the vertical transmission of SIV in sooty mangabeys.

Sat Mar 01 00:00:00 EST 2014

The exonuclease domain of Lassa virus nucleoprotein is involved in antigen-presenting-cell-mediated NK cell responses.

Journal of virology

Russier M,Reynard S,Carnec X,Baize S

15-0959 was used in Flow cytometry/Cell sorting to demonstrate for the first time that Lassa virus nucleoprotein is involved in the inhibition of antigen-presenting cell-mediated natural killer cell responses.

Mon Dec 01 00:00:00 EST 2014

Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways.

Molecular medicine reports

Shi LS,Wang H,Wang F,Feng M,Wang M,Guan WX

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Dompe N,Rivers CS,Li L,Cordes S,Schwickart M,Punnoose EA,Amler L,Seshagiri S,Tang J,Modrusan Z,Davis DP

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Tue Oct 25 00:00:00 EDT 2011

CD40·FasL and CTLA-4·FasL fusion proteins induce apoptosis in malignant cell lines by dual signaling.

The American journal of pathology

Orbach A,Rachmilewitz J,Shani N,Isenberg Y,Parnas M,Huang JH,Tykocinski ML,Dranitzki-Elhalel M

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 15-0959-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Dog) 486469, (Human) 355

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Function(s)

protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD95 (APO-1/Fas) Monoclonal Antibody (DX2), PE-Cyanine5, eBioscience™

CD95 (APO-1/Fas) Antibody (15-0959-42) in Flow

Product Details

15-0959-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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