Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (SM 1-13), eBioscience™

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Protocols
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Product Details

BMS1030

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-Dependent
-

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SM 1-13

Immunogen

Rec. Human CD95 (APO-1/Fas)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10596849

Product Specific Information

Description: APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO-1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system.

Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes.

Applications Tested: ELISA, Flow Cytometry.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Human) 355

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Function(s)
protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor
Process(es)
ovarian follicle atresia immunoglobulin production renal system process protein complex assembly apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process inflammatory response immune response signal transduction spermatogenesis brain development aging circadian rhythm extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression B cell mediated immunity telencephalon development dendrite regeneration positive regulation of protein homooligomerization response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation response to drug ovulation cycle regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process negative regulation of apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to peptide hormone negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation response to cycloheximide spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid maternal process involved in female pregnancy positive regulation of lymphocyte apoptotic process cellular response to hydrogen peroxide response to growth factor cellular response to phenylalanine cellular response to mechanical stimulus cellular response to cobalt ion cellular response to lithium ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to estrogen stimulus cellular response to hyperoxia cellular response to hypoxia cellular response to hydrostatic pressure motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway liver regeneration necroptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors response to fluoride positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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