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CD96 Chimeric Antibody (MA5-48214) in ELISA
ELISA analysis of CD96 chimeric monoclonal antibody (Product # MA5-48214) (blue line) was performed by coating plate with CD96-Fc fusion protein at a concentration of 5 µg/mL. A 3-fold serial dilution from 10,000 to 1.7 ng/mL of the antibody was done. For signal detection, a 1:4,000 dilution of anti-rabbit IgG1 HRP antibody was used.
Product Details
MA5-48214
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6A6
Immunogen
This antibody was raised by immunising rats with murine CD96.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074657
Product Specific Information
Specificity: This antibody is specific for murine CD96, and recognises an epitope in the first Ig domain.
Target Information
CD96 is a cell adhesion receptor also known as TACTILE. A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region. CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells). In the mouse, this receptor is not expressed on B cells. Unlike in humans, mouse CD96 does not exist as alternative splice variants. Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD96; Cell surface antigen CD96; DKFZp667E2122; sCD96; soluble CD96; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile
Gene Aliases: 1700109I12Rik; Cd96; Tactile
UniProt ID: (Mouse) Q3U0X8
Entrez Gene ID: (Mouse) 84544
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