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Invitrogen
CD96 (TACTILE) Monoclonal Antibody (NK92.39), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 2
CD96 (TACTILE) Antibody (46-0969-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD96 (TACTILE) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
46-0969-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NK92.39
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573702
Product Specific Information
Description: The NK92.39.1 monoclonal antibody reacts with human CD96 also known as TACTILE (T cells-activated increased late expression). CD96 is a type I membrane protein of the Immunoglobulin Superfamily. CD96 promotes NK cell adhesion to cells expressing the polio virus receptor (CD155). Therefore CD96 is an additional receptor for CD155. After binding of PVR to CD96, the PVR can be transferred to the target cell and CD96 can be internalized with bound PVR. This internalization may lead to cell death of the NK cell. This phenomenon may be significant in tumor cells which express high levels of PVR. Furthermore expression of CD96 has been found in the majority of CD34(+)CD38(-) Acute Myeloid Leukemic cells.)
The NK92.39.1 antibody can block binding of soluble CD155 to NK cell lines.
Applications Reported: This NK92.39 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This NK92.39 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD96 is a cell adhesion receptor also known as TACTILE. A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region. CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells). In the mouse, this receptor is not expressed on B cells. Unlike in humans, mouse CD96 does not exist as alternative splice variants. Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD96; Cell surface antigen CD96; DKFZp667E2122; sCD96; soluble CD96; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile
Gene Aliases: CD96; TACTILE
UniProt ID: (Human) P40200
Entrez Gene ID: (Human) 10225
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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