Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
CD97 Antibody (PA5-148204) in WB
Western blot analysis of CD97 in various lysates. Samples were incubated with CD97 polyclonal antibody (Product # PA5-148204) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG (H+L)/HRP at a dilution of 1:10,000. Lane A: NIH/3T3 Whole Cell Lysate; Lane B: HepG2 Whole Cell Lysate; Lane C: Raw264.7 Whole Cell Lysate; Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 μg per lane. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa .
Product Details
PA5-148204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Mouse CD97/ADGRE5
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E5; CD97; CD97 antigen; EGF-TM7 receptor; Leukocyte antigen CD97
Gene Aliases: AA409984; Adgre5; Cd97; TM7LN1
UniProt ID: (Mouse) Q9Z0M6
Entrez Gene ID: (Mouse) 26364
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support