Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Description: The 3B2/TA8 monoclonal antibody reacts with human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation.
Applications Reported: The 3B2/TA8 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3B2/TA8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: 12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; MIC2 (monoclonal antibody 12E7); Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2
Gene Aliases: CD99; HBA71; MIC2; MIC2X; MIC2Y; MSK5X
UniProt ID: (Human) P14209
Entrez Gene ID: (Human) 4267
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