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Product Details
MA5-47703
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M030
Immunogen
Clone M030 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.
Conjugate
Form
Liquid
Concentration
~0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942684
Product Specific Information
Clone M030 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.
Target Information
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; MIC2 (monoclonal antibody 12E7); Paired immunoglobin-like type 2 receptor-ligand; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2
Gene Aliases: 1110061M03Rik; 2410026K10Rik; CD99; D4; HBA71; MIC2; MIC2X; MIC2Y; MSK5X; pilr-1; Pilr-l; Pilrl
UniProt ID: (Human) P14209, (Mouse) Q8VCN6
Entrez Gene ID: (Human) 4267, (Rat) 652929, (Mouse) 673094
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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