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Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is pancreas tissue.
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
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Protein Aliases: 12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; MIC2 (monoclonal antibody 12E7); Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2
Gene Aliases: CD99; HBA71; MIC2; MIC2X; MIC2Y; MSK5X
UniProt ID: (Human) P14209
Entrez Gene ID: (Human) 4267
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