Proteintech

CDC6 Polyclonal Antibody

CDC6 Antibody in Immunocytochemistry (ICC/IF)

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CDC6 Antibody (11640-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using CDC6 antibody 11640-1-AP at 1:50 and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CDC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

11640-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CDC6 Fusion Protein Ag2228 (217-560 aa encoded by BC025232)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Immunogen sequence: QDLKKELKG FKTIMLNCMS LRTAQAVFPA IAQEICQEEV SRPAGKDMMR KLEKHMTAEK GPMIVLVLDE MDQLDSKGQD VLYTLFEWPW LSNSHLVLIG IANTLDLTDR ILPRLQAREK CKPQLLNFPP YTRNQIVTIL QDRLNQVSRD QVLDNAAVQF CARKVSAVSG DVRKALDVCR RAIEIVESDV KSQTILKPLS ECKSPSEPLI PKRVGLIHIS QVISEIDGNR MTLSQEGAQD SFPLQQKILV CSLMLLIRQL KIKEVTLGKL YEAYSKVCRK QQVAAVDQSE CLSLSGLLEA RGILGLKRNK ETRLTKVFFK IEEKEIEHAL KDKALIGNIL ATGLP (217-560 aa encoded by BC025232)

Target Information

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells.

Cell death & disease

Wang R,Wen P,Yang G,Feng Y,Mi Y,Wang X,Zhu S,Chen YQ

Tue Jul 19 00:00:00 EDT 2022

Norcantharidin Induces Immunogenic Cell Death of Bladder Cancer Cells through Promoting Autophagy in Acidic Culture.

International journal of molecular sciences

Xu L,Su B,Mo L,Zhao C,Zhao Z,Li H,Hu Z,Li J

Fri Apr 01 00:00:00 EDT 2022

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 EDT 2022

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 EDT 2022

Heat Stress Modulates a Placental Immune Response Associated With Alterations in the Development of the Fetal Intestine and Its Innate Immune System in Late Pregnant Mouse.

Frontiers in physiology

Guo H,Liu R,He J,Yao W,Zheng W

Sat Jul 16 00:00:00 EDT 2022

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.

Aging

Zou K,Li Z,Zhang Y,Mu L,Chen M,Wang R,Deng W,Zou L,Liu J

Wed Dec 09 00:00:00 EST 2020

A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.

Molecular cancer

He X,Chai P,Li F,Zhang L,Zhou C,Yuan X,Li Y,Yang J,Luo Y,Ge S,Zhang H,Jia R,Fan X

Wed Jul 15 00:00:00 EDT 2020

Circular RNA circNELL2 Acts as the Sponge of miR-127-5p to Promote Esophageal Squamous Cell Carcinoma Progression.

OncoTargets and therapy

Xiong G,Diao D,Lu D,Liu X,Liu Z,Mai S,Feng S,Dong X,Cai K

Sun Apr 17 00:00:00 EDT 2022

Interactome analysis of gene expression profiles identifies CDC6 as a potential therapeutic target modified by miR-215-5p in hepatocellular carcinoma.

International journal of medical sciences

Xu H,Huang J,Hua S,Liang L,He X,Zhan M,Lu L,Chu J

Mon Aug 02 00:00:00 EDT 2021

Phenotypic transition of tumor cells between epithelial- and mesenchymal-like state during adaptation to acidosis.

Cell cycle (Georgetown, Tex.)

Zhang Y,Xu L,Wang P,Jian H,Shi X,Jia M,Mo L,Hu Z,Li H,Li J

Thu Aug 01 00:00:00 EDT 2019

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 EDT 2017

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 EDT 2017

PRMT5 competitively binds to CDK4 to promote G1-S transition upon glucose induction in hepatocellular carcinoma.

Oncotarget

Yang H,Zhao X,Zhao L,Liu L,Li J,Jia W,Liu J,Huang G

Tue Nov 01 00:00:00 EDT 2016

PRMT5 competitively binds to CDK4 to promote G1-S transition upon glucose induction in hepatocellular carcinoma.

Oncotarget

Yang H,Zhao X,Zhao L,Liu L,Li J,Jia W,Liu J,Huang G

Tue Nov 01 00:00:00 EDT 2016

YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers.

Genes to cells : devoted to molecular & cellular mechanisms

Harada M,Kotake Y,Ohhata T,Kitagawa K,Niida H,Matsuura S,Funai K,Sugimura H,Suda T,Kitagawa M

Sun Jun 01 00:00:00 EDT 2014

YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers.

Genes to cells : devoted to molecular & cellular mechanisms

Harada M,Kotake Y,Ohhata T,Kitagawa K,Niida H,Matsuura S,Funai K,Sugimura H,Suda T,Kitagawa M

Sun Jun 01 00:00:00 EDT 2014

Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.

PLoS genetics

Ma P,Schultz RM

Thu Jun 13 00:00:00 EDT 2013

Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.

PLoS genetics

Ma P,Schultz RM

Thu Jun 13 00:00:00 EDT 2013

N-myc downstream regulated gene1/Cap43 overexpression suppresses tumor growth by hepatic cancer cells through cell cycle arrest at the G0/G1 phase.

Cancer letters

Akiba J,Murakami Y,Noda M,Watari K,Ogasawara S,Yoshida T,Kawahara A,Sanada S,Yasumoto M,Yamaguchi R,Kage M,Kuwano M,Ono M,Yano H

Tue Nov 01 00:00:00 EDT 2011

N-myc downstream regulated gene1/Cap43 overexpression suppresses tumor growth by hepatic cancer cells through cell cycle arrest at the G0/G1 phase.

Cancer letters

Akiba J,Murakami Y,Noda M,Watari K,Ogasawara S,Yoshida T,Kawahara A,Sanada S,Yasumoto M,Yamaguchi R,Kage M,Kuwano M,Ono M,Yano H

Tue Nov 01 00:00:00 EDT 2011

Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.

Proceedings of the National Academy of Sciences of the United States of America

Yim H,Erikson RL

Tue Nov 16 00:00:00 EST 2010

Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.

Proceedings of the National Academy of Sciences of the United States of America

Yim H,Erikson RL

Tue Nov 16 00:00:00 EST 2010

Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.

European journal of cancer (Oxford, England : 1990)

Basaki Y,Taguchi K,Izumi H,Murakami Y,Kubo T,Hosoi F,Watari K,Nakano K,Kawaguchi H,Ohno S,Kohno K,Ono M,Kuwano M

Mon Mar 01 00:00:00 EST 2010

Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.

European journal of cancer (Oxford, England : 1990)

Basaki Y,Taguchi K,Izumi H,Murakami Y,Kubo T,Hosoi F,Watari K,Nakano K,Kawaguchi H,Ohno S,Kohno K,Ono M,Kuwano M

Mon Mar 01 00:00:00 EST 2010

Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.

European journal of cancer (Oxford, England : 1990)

Basaki Y,Taguchi K,Izumi H,Murakami Y,Kubo T,Hosoi F,Watari K,Nakano K,Kawaguchi H,Ohno S,Kohno K,Ono M,Kuwano M

Mon Mar 01 00:00:00 EST 2010

Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.

European journal of cancer (Oxford, England : 1990)

Basaki Y,Taguchi K,Izumi H,Murakami Y,Kubo T,Hosoi F,Watari K,Nakano K,Kawaguchi H,Ohno S,Kohno K,Ono M,Kuwano M

Mon Mar 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CDC18(S.pombe); Cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; Cell division control protein 6 homolog; cell division cycle 6 homolog; HsCdc18; HsCDC6; p62(cdc6)

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Gene Aliases: CDC18L; CDC6; HsCDC18; HsCDC6

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UniProt ID: (Human) Q99741, (Mouse) O89033

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Entrez Gene ID: (Human) 990, (Mouse) 23834, (Rat) 360621

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Function(s)

nucleotide binding protein binding ATP binding kinase binding chromatin binding replication origin binding protein

Process(es)

DNA replication checkpoint regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle DNA replication DNA replication initiation mitotic nuclear division traversing start control point of mitotic cell cycle negative regulation of DNA replication negative regulation of cell proliferation regulation of mitotic metaphase/anaphase transition positive regulation of cytokinesis positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of fibroblast proliferation cell division positive regulation of chromosome segregation cellular response to vasopressin cellular response to angiotensin cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDC6 Polyclonal Antibody

CDC6 Antibody (11640-1-AP) in ICC/IF

Product Details

11640-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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