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CDH17 Antibody (MA5-29136) in ICC/IF
Immunofluorescence analysis of Cadherin-17 was performed using 70% confluent log phase Caco-2 and SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDH17 Recombinant Rabbit Monoclonal Antibody (Product # MA5-29136) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa ... View More
Product Details
MA5-29136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
029
Immunogen
Recombinant Human Cadherin-17/LI-cadherin/CDH17 protein (Met1-Met787)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785059
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Cadherin-17/LI-cadherin/CDH17.
Target Information
CDH17 gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cadherin; cadherin 17, LI cadherin (liver-intestine); cadherin-16; Cadherin-17; FLJ26931; HPT-1 cadherin; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024
Gene Aliases: CDH16; CDH17; HPT-1; HPT1
UniProt ID: (Human) Q12864
Entrez Gene ID: (Human) 1015
germinal center B cell differentiation
marginal zone B cell differentiation
transport
oligopeptide transport
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
integrin-mediated signaling pathway
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
positive regulation of integrin activation by cell surface receptor linked signal transduction
adherens junction organization
oligopeptide transmembrane transport
spleen development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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