OriGene

CDH2 Monoclonal Antibody (OTI2G7), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (97) N-cadherin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF503933

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Dog, Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI2G7

Immunogen

Full length human recombinant protein of human CDH2 produced in HEK293T cell.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116

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Entrez Gene ID: (Dog) 480169, (Human) 1000, (Mouse) 12558

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Function(s)
calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding cadherin
Process(es)
cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering synapse assembly regulation of signal transduction single organismal cell-cell adhesion regulation of protein localization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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