Proteintech

CDK1 Polyclonal Antibody

CDK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

CDK1 Antibody (10762-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 10762-1-AP) (CDK1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

10762-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 44 publications 44 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

1:50

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CDK1 Fusion Protein Ag1183 (1-297 aa encoded by BC014563)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEDYTKIEK IGEGTYGVVY KGRHKTTGQV VAMKKIRLES EEEGVPSTAI REISLLKELR HPNIVSLQDV LMQDSRLYLI FEFLSMDLKK YLDSIPPGQY MDSSLVKSYL YQILQGIVFC HSRRVLHRDL KPQNLLIDDK GTIKLADFGL ARAFGIPIRV YTHEVVTLWY RSPEVLLGSA RYSTPVDIWS IGTIFAELAT KKPLFHGDSE IDQLFRIFRA LGTPNNEVWP EVESLQDYKN TFPKWKPGSL ASHVKNLDEN GLDLLSKMLI YDPAKRISGK MALNHPYFND LDNQIKKM (1-297 aa encoded by BC014563)

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase

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Gene Aliases: CDC2; CDC28A; CDK1; CDKN1; P34CDC2

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UniProt ID: (Human) P06493

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Entrez Gene ID: (Human) 983

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Function(s)

protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity cyclin binding Hsp70 protein binding histone kinase activity non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division mitotic nuclear envelope disassembly mitotic G2 DNA damage checkpoint centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus

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CDK1 Polyclonal Antibody

CDK1 Antibody (10762-1-AP) in ICC/IF

Product Details

10762-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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