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CDK1 Antibody (PA5-77900) in ICC/IF
CDK1 Polyclonal Antibody detects CDC2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min. Green: CDC2 protein stained by CDK1 Polyclonal Antibody (Product # PA5-77900) diluted at 1:750. Blue: Hoechst 33342 staining.
Product Details
PA5-77900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDC2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 0.1M glycine, 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735615
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, NIH-3T3, JC, BCL-1, PC-12
Predicted Reactivity: Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase
Gene Aliases:
CDC2; CDC28A; Cdc2a; CDK1; CDKN1; p34
UniProt ID: (Human) P06493, (Rat) P39951, (Mouse) P11440
Entrez Gene ID: (Human) 983, (Rat) 54237, (Mouse) 12534
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
cyclin binding
Hsp70 protein binding
histone kinase activity
kinase activity
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
activation of MAPK activity
microtubule cytoskeleton organization
DNA replication
DNA repair
protein complex assembly
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic G2 DNA damage checkpoint
centrosome cycle
pronuclear fusion
cell aging
response to toxic substance
positive regulation of gene expression
regulation of Schwann cell differentiation
response to organic cyclic compound
response to amine
response to activity
cell migration
histone phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
chromosome condensation
epithelial cell differentiation
organ regeneration
anaphase-promoting complex-dependent catabolic process
positive regulation of protein import into nucleus, translocation
protein localization to kinetochore
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
response to ethanol
positive regulation of DNA replication
positive regulation of mitotic cell cycle
regulation of embryonic development
response to cadmium ion
response to copper ion
response to axon injury
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
cellular response to hydrogen peroxide
Golgi disassembly
positive regulation of protein localization to nucleus
mitotic cell cycle
protein phosphorylation
response to organonitrogen compound
response to hydrogen peroxide
cell cycle
phosphorylation
Performance Guarantee
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