Invitrogen

CDK1 Monoclonal Antibody (A17.1.1)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

21 References

View all (88) CDK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CDK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

CDK1 Antibody (MA5-11472) in ICC/IF

Immunofluorescent analysis of Cdk1 (green) showing staining in the cytoplasm of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk1 monoclonal antibody (Product # MA5-11472) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-acti... View More

CDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDK1 Antibody in Immunoprecipitation (IP)

CDK1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CDK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CDK1 siRNA and reduction of signal was observed in Western Blot using CDK1 Monoclonal Antibody (Product # MA5-11472). {KD}

Product Details

MA5-11472

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mg protein lysate

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Guinea pig, Human, Mouse, Rodent, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

A17.1.1

Immunogen

C-Terminal 2/3 of Xenopus CDC2 expressed in E. coli

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979734

Product Specific Information

MA5-11472 targets Cdk1 in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Chicken, Guinea Pig, Human, mouse, Rodent, Rat, and Xenopus laevis samples.

The MA5-11472 immunogen is c-Terminal 2/3 of Xenopus CDC2 expressed in E. coli.

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Oligomeric Assemblies of Cytomegalovirus Core Nuclear Egress Proteins Are Associated with Host Kinases and Show Sensitivity to Antiviral Kinase Inhibitors.

Viruses

Kicuntod J,Häge S,Hahn F,Sticht H,Marschall M

MA5-11472 was used in PCR to underline the functional importance of the oligomerization of the HCMV-specific NEC that is both phosphorylation-dependent and sensitive to antiviral kinase inhibitors.

Wed May 11 00:00:00 EDT 2022

Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G₂ Arrest and Highly Effective Evasion of Innate Immune Sensing.

Journal of virology

Caller LG,Davies CTR,Antrobus R,Lehner PJ,Weekes MP,Crump CM

Published figure using CDK1 monoclonal antibody (Product # MA5-11472) in Western Blot

Thu Aug 15 00:00:00 EDT 2019

Pharmaceutically inhibiting polo-like kinase 1 exerts a broad anti-tumour activity in retinoblastoma cell lines.

Clinical & experimental ophthalmology

Schwermer M,Dreesmann S,Eggert A,Althoff K,Steenpass L,Schramm A,Schulte JH,Temming P

MA511472 was used in western blot to use polo-like kinase 1 inhibitors for a rationally designed chemotherapy protocols to treat patients with metastasized retinoblastoma

Sat Apr 01 00:00:00 EDT 2017

Pharmaceutically inhibiting polo-like kinase 1 exerts a broad anti-tumour activity in retinoblastoma cell lines.

Clinical & experimental ophthalmology

Schwermer M,Dreesmann S,Eggert A,Althoff K,Steenpass L,Schramm A,Schulte JH,Temming P

MA511472 was used in western blot to use polo-like kinase 1 inhibitors for a rationally designed chemotherapy protocols to treat patients with metastasized retinoblastoma

Sat Apr 01 00:00:00 EDT 2017

IFI27, a novel epidermal growth factor-stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes.

Cell proliferation

Hsieh WL,Huang YH,Wang TM,Ming YC,Tsai CN,Pang JH

MA5-11472 was used in western blot to identify the role of IFI27 in the cell proliferation of human epidermal keratinocytes

Wed Apr 01 00:00:00 EDT 2015

Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.

Oncotarget

Zhang Y,Yu J,Lee C,Xu B,Sartor MA,Koenig RJ

MA5-11472 was used in western blot to characterize the thyroid carcinoma-associated PAX8-PPARG fusion protein and genomic binding and regulation of gene expression

Tue Dec 01 00:00:00 EST 2015

Cyclin C is a haploinsufficient tumour suppressor.

Nature cell biology

Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P

MA5-11472 was used in immunoprecipitation and western blot to elucidate the regulation of Notch by Cyclin C.

Sat Nov 01 00:00:00 EDT 2014

Cyclin C is a haploinsufficient tumour suppressor.

Nature cell biology

MA5-11472 was used in immunoprecipitation and western blot to elucidate the regulation of Notch by Cyclin C.

Sat Nov 01 00:00:00 EDT 2014

The cyclin D1-CDK4 oncogenic interactome enables identification of potential novel oncogenes and clinical prognosis.

Cell cycle (Georgetown, Tex.)

Jirawatnotai S,Sharma S,Michowski W,Suktitipat B,Geng Y,Quackenbush J,Elias JE,Gygi SP,Wang YE,Sicinski P

MA5-11472 was used in western blot to identify CDK4 binding partners.

Thu Aug 20 00:00:00 EDT 2015

Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.

PloS one

Silva AR,Santos AC,Farfel JM,Grinberg LT,Ferretti RE,Campos AH,Cunha IW,Begnami MD,Rocha RM,Carraro DM,de Bragança Pereira CA,Jacob-Filho W,Brentani H

MA5-11472 was used in immunohistochemistry - paraffin section to study the clinical manifestation of Alzheimer's disease involving cell-cycle regulation, neuronal death and repair of oxidative DNA damage

Thu Feb 12 00:00:00 EST 2015

Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.

PloS one

Silva AR,Santos AC,Farfel JM,Grinberg LT,Ferretti RE,Campos AH,Cunha IW,Begnami MD,Rocha RM,Carraro DM,de Bragança Pereira CA,Jacob-Filho W,Brentani H

Thu Feb 12 00:00:00 EST 2015

Activated Akt as an indicator of prognosis in gastric cancer.

Virchows Archiv : an international journal of pathology

Cinti C,Vindigni C,Zamparelli A,La Sala D,Epistolato MC,Marrelli D,Cevenini G,Tosi P

MA5-11472 was used in immunohistochemistry to examine the prognostic significance of activated AKT in gastric cancer

Sat Nov 01 00:00:00 EDT 2008

Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.

The FEBS journal

Choi JK,Murillo G,Su BN,Pezzuto JM,Kinghorn AD,Mehta RG

MA5-11472 was used in western blot to study the antiproliferative and apoptotic effects of ixocarpalactone A on colon cancer cells

Fri Dec 01 00:00:00 EST 2006

In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.

Clinical cancer research : an official journal of the American Association for Cancer Research

Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P

MA5-11472 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma

Fri Jul 01 00:00:00 EDT 2005

Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.

Human pathology

Nakamura N,Yamamoto H,Yao T,Oda Y,Nishiyama K,Imamura M,Yamada T,Nawata H,Tsuneyoshi M

MA5-11472 was used in immunohistochemistry to study the prognostic value of the expression of a range of cell cycle regulatory proteins in gastrointestinal stromal tumors

Fri Jul 01 00:00:00 EDT 2005

Mitotic cell cycle proteins increase in podocytes despite lack of proliferation.

Kidney international

Petermann AT,Pippin J,Hiromura K,Monkawa T,Durvasula R,Couser WG,Kopp J,Shankland SJ

MA5-11472 was used in immunocytochemistry to study the increase in mitotic cell cycle protein in podocyes in the absence of increased proliferation

Wed Jan 01 00:00:00 EST 2003

c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.

British journal of cancer

Sheen JH,Woo JK,Dickson RB

MA5-11472 was used in western blot to study the effect of c-Myc on DNA damage-induced G2/M arrest.

Mon Oct 20 00:00:00 EDT 2003

A novel, extraneuronal role for cyclin-dependent protein kinase 5 (CDK5): modulation of cAMP-induced apoptosis in rat leukemia cells.

The Journal of biological chemistry

Sandal T,Stapnes C,Kleivdal H,Hedin L,Døskeland SO

MA5-11472 was used in western blot to study the role cyclin-dependent protein kinase 5 in modulating cAMP-induced apoptosis in rat leukemia cells

Fri Jun 07 00:00:00 EDT 2002

Expression of the ALK protein by anaplastic large-cell lymphomas correlates with high proliferative activity.

International journal of cancer

Leoncini L,Lazzi S,Scano D,Mura A,Onida A,Massarelli G,Tosi P,Barbini P,Cevenini G,Massai MR,Pileri S,Falini B,Giordano A,Kraft R,Laissue JA,Cottier H

MA5-11472 was used in immunohistochemistry to study the correlation between ALK protein expression and the proliferative capacity of anaplastic large-cell lymphomas

Thu Jun 15 00:00:00 EDT 2000

Retinoblastoma-related p107 and pRb2/p130 proteins in malignant lymphomas: distinct mechanisms of cell growth control.

Clinical cancer research : an official journal of the American Association for Cancer Research

Leoncini L,Bellan C,Cossu A,Claudio PP,Lazzi S,Cinti C,Cevenini G,Megha T,Laurini L,Luzi P,Orcioni GF,Piccioli M,Pileri S,Giardino C,Tosi P,Giordano A

MA5-11472 was used in immunohistochemistry to study the expression and role of retinoblastoma protein/p107 and retinoblastoma protein2/p130 in non-Hodgkin's lymphoma

Wed Dec 01 00:00:00 EST 1999

Expression of p34(cdc2) and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: a multivariate cluster analysis.

International journal of cancer

Leoncini L,Cossu A,Megha T,Bellan C,Lazzi S,Luzi P,Tosi P,Barbini P,Cevenini G,Pileri S,Giordano A,Kraft R,Laissue JA,Cottier H

MA5-11472 was used in immunohistochemistry to perform a multivariate cluster analysis of p34(cdc2), cyclin A and cyclin B expresssion in non-Hodgkin's lymphoma

Fri Oct 08 00:00:00 EDT 1999

Dissociation between cell cycle arrest and apoptosis can occur in Li-Fraumeni cells heterozygous for p53 gene mutations.

Oncogene

Delia D,Goi K,Mizutani S,Yamada T,Aiello A,Fontanella E,Lamorte G,Iwata S,Ishioka C,Krajewski S,Reed JC,Pierotti MA

MA5-11472 was used in Western Blotting to show that, in cells harbouring heterozygous mutations of p53, the G1 checkpoint is not necessarily disrupted, and this may be related to the endogenous p53 heterocomplexes having lost or not the capacity to bind DNA.

Thu May 08 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: ABL1; CDK1; CDK1-A; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; DKFZp686L20222; maturation-promoting factor; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; unnamed protein product

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Gene Aliases: cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; cdk1-b; CDKN1; MPF; p34; P34CDC2; PSTAIR; xcdc2; XELAEV_18036802mg

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UniProt ID: (Chicken) P13863, (Human) P06493, (Rat) P39951, (Mouse) P11440, (Xenopus) P35567

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Entrez Gene ID: (Chicken) 396252, (Human) 983, (Guinea pig) 100735946, (Rat) 54237, (Mouse) 12534, (Xenopus) 380246

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Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity protein kinase activity protein binding cyclin binding Hsp70 protein binding histone kinase activity kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G2/M transition of mitotic cell cycle double-strand break repair via homologous recombination protein phosphorylation mitotic G2 DNA damage checkpoint cellular response to heat regulation of circadian rhythm rhythmic process cell division G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division mitotic nuclear envelope disassembly centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus mitotic cell cycle response to organonitrogen compound response to hydrogen peroxide cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK1 Monoclonal Antibody (A17.1.1)

CDK1 Antibody (MA5-11472) in ICC/IF

Product Details

MA5-11472

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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