Invitrogen

CDK1 Monoclonal Antibody (A17)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

11 References

View all (88) CDK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

CDK1 Antibody (33-1800) in IHC (P)

Immunohistochemistry analysis of CDC2/CDK1 showing staining in the cytoplasm and membrane of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15... View More

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CDK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with CDK1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using Anti-CDK1 Mouse Monoclonal Antibody (Product # 33-1800). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

Product Details

33-1800

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunohistochemistry (Frozen) (IHC (F))

5-10 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

- -

Immunoprecipitation (IP)

5 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Amphibian, Chicken, Human, Mouse, Rat, Xenopus

Published species

Human, Mouse, Xenopus

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

A17

Immunogen

Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533105

Target Information

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition.

Nature

Gallo D,Young JTF,Fourtounis J,Martino G,Álvarez-Quilón A,Bernier C,Duffy NM,Papp R,Roulston A,Stocco R,Szychowski J,Veloso A,Alam H,Baruah PS,Fortin AB,Bowlan J,Chaudhary N,Desjardins J,Dietrich E,Fournier S,Fugère-Desjardins C,Goullet de Rugy T,Leclaire ME,Liu B,Bhaskaran V,Mamane Y,Melo H,Nicolas O,Singhania A,Szilard RK,Tkáč J,Yin SY,Morris SJ,Zinda M,Marshall CG,Durocher D

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Fri Apr 01 00:00:00 EDT 2022

Downregulation of Stem-Loop Binding Protein by Nicotine via α7-Nicotinic Acetylcholine Receptor and Its Role in Nicotine-Induced Cell Transformation.

Toxicological sciences : an official journal of the Society of Toxicology

Sun Q,Chen D,Raja A,Grunig G,Zelikoff J,Jin C

33-1800 was used in Western Blotting to propose that the SLBP depletion and polyadenylation of canonical histone mRNAs via activation of α7-nAChR and a series of downstream signal transduction pathways are critical for nicotine-induced cell transformation and potential carcinogenesis.

Sat Sep 24 00:00:00 EDT 2022

Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement.

Nature communications

Liu J,Wang Y,Mu C,Li M,Li K,Li S,Wu W,Du L,Zhang X,Li C,Peng W,Shen J,Liu Y,Yang D,Zhang K,Ning Q,Fu X,Zeng Y,Ni Y,Zhou Z,Liu Y,Hu Y,Zheng X,Wen T,Li Z,Liu Y

33-1800 was used in Western Blot experiments to formulate a DNA-barcoded micellular system (DMS) encapsulating the Pin1 inhibitor AG17724.

Mon Jul 25 00:00:00 EDT 2022

Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Scientific reports

Moretti FA,Giardino G,Attenborough TCH,Gkazi AS,Margetts BK,la Marca G,Fairbanks L,Crompton T,Gaspar HB

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.

Aging

Jeon S,Yoon YS,Kim HK,Han J,Lee KM,Seol JE,Cho SK,Park CS

33-1800 was used in Western Blotting to demonstrate reversion of the decreased protein level of collagen I after the reintroduction of the CRBN gene back into CRBN KO MEFs.

Wed Mar 03 00:00:00 EST 2021

Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.

Aging

Jeon S,Yoon YS,Kim HK,Han J,Lee KM,Seol JE,Cho SK,Park CS

33-1800 was used in Western Blotting to demonstrate reversion of the decreased protein level of collagen I after the reintroduction of the CRBN gene back into CRBN KO MEFs.

Wed Mar 03 00:00:00 EST 2021

PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer.

Cancer cell international

Chai Z,Wang L,Zheng Y,Liang N,Wang X,Zheng Y,Zhang Z,Zhao C,Zhu T,Liu C

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Sat Nov 04 00:00:00 EDT 2023

Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - A model of kinase-dependent regulation of protein expression.

Molecular and cellular endocrinology

Miedlich SU,Taya M,Young MR,Hammes SR

33-1800 was used in Western Blotting to study whether paxillin enhances Mos translation by interacting with embryonic PolyAdenylation Binding Protein on polyadenylated Mos mRNA.

Thu Jun 15 00:00:00 EDT 2017

Enhanced expression of mitochondrial superoxide dismutase leads to prolonged in vivo cell cycle progression and up-regulation of mitochondrial thioredoxin.

Free radical biology & medicine

Kim A,Joseph S,Khan A,Epstein CJ,Sobel R,Huang TT

33-1800 was used in immunohistochemistry - paraffin section to investigate the role of Mn superoxide dismutase in tissue regeneration using transgenic mice

Tue Jun 01 00:00:00 EDT 2010

Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis.

The Journal of urology

Hauser PJ,Dozmorov MG,Bane BL,Slobodov G,Culkin DJ,Hurst RE

33-1800 was used in Immunohistochemistry to determine the origins of the loss of the glycosaminoglycan layer and investigate more fully the altered differentiation of the urothelium in interstitial cystitis.

Fri Feb 01 00:00:00 EST 2008

CDC2/CDK1 expression in esophageal adenocarcinoma and precursor lesions serves as a diagnostic and cancer progression marker and potential novel drug target.

The American journal of surgical pathology

Hansel DE,Dhara S,Huang RC,Ashfaq R,Deasel M,Shimada Y,Bernstein HS,Harmon J,Brock M,Forastiere A,Washington MK,Maitra A,Montgomery E

Tue Mar 01 00:00:00 EST 2005

Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration.

The Journal of cell biology

Manes T,Zheng DQ,Tognin S,Woodard AS,Marchisio PC,Languino LR

Mon May 26 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: ABL1; CDK1; CDK1-A; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; DKFZp686L20222; maturation-promoting factor; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; unnamed protein product

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Gene Aliases: cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; cdk1-b; CDKN1; MPF; p34; P34CDC2; PSTAIR; xcdc2; XELAEV_18036802mg

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UniProt ID: (Chicken) P13863, (Human) P06493, (Rat) P39951, (Mouse) P11440, (Xenopus) P35567

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Entrez Gene ID: (Chicken) 396252, (Human) 983, (Rat) 54237, (Mouse) 12534, (Xenopus) 380246

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Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity protein kinase activity protein binding cyclin binding Hsp70 protein binding histone kinase activity kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G2/M transition of mitotic cell cycle double-strand break repair via homologous recombination protein phosphorylation mitotic G2 DNA damage checkpoint cellular response to heat regulation of circadian rhythm rhythmic process cell division G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle activation of MAPK activity microtubule cytoskeleton organization DNA replication DNA repair protein complex assembly apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division mitotic nuclear envelope disassembly centrosome cycle pronuclear fusion cell aging response to toxic substance positive regulation of gene expression regulation of Schwann cell differentiation response to organic cyclic compound response to amine response to activity cell migration histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation organ regeneration anaphase-promoting complex-dependent catabolic process positive regulation of protein import into nucleus, translocation protein localization to kinetochore response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process response to ethanol positive regulation of DNA replication positive regulation of mitotic cell cycle regulation of embryonic development response to cadmium ion response to copper ion response to axon injury negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation cellular response to hydrogen peroxide Golgi disassembly positive regulation of protein localization to nucleus mitotic cell cycle response to organonitrogen compound response to hydrogen peroxide cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK1 Monoclonal Antibody (A17)

CDK1 Antibody (33-1800) in IHC (P)

Product Details

33-1800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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