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OriGene
CDK2 Monoclonal Antibody (OTI2D9), TrueMAB™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Product Details
CF502935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2D9
Immunogen
Full length human recombinant protein of human CDK2 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2
Gene Aliases: CDK2; CDKN2; p33(CDK2)
UniProt ID: (Human) P24941
Entrez Gene ID: (Dog) 100855704, (Human) 1017, (Rat) 362817
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
lens development in camera-type eye
DNA replication
DNA repair
potassium ion transport
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic nuclear division
centriole replication
meiotic nuclear division
Ras protein signal transduction
positive regulation of cell proliferation
response to toxic substance
histone phosphorylation
peptidyl-serine phosphorylation
organ regeneration
mitotic G1 DNA damage checkpoint
positive regulation of DNA-dependent DNA replication initiation
response to estradiol
cellular response to insulin stimulus
response to drug
response to ethanol
positive regulation of transcription, DNA-templated
response to cadmium ion
centrosome duplication
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
response to cAMP
response to electrical stimulus
regulation of gene silencing
cellular response to nitric oxide
regulation of signal transduction by p53 class mediator
protein phosphorylation
meiotic cell cycle
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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