Proteintech

CDK4 Monoclonal Antibody (1G2C12)

CDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDK4 Antibody (66950-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

66950-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G2C12

Immunogen

CDK4 Fusion Protein Ag20538

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Thu Jun 01 00:00:00 EDT 2023

NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer.

Journal of gastrointestinal oncology

Yang H,Pu L,Li R,Zhu R

Sat Apr 29 00:00:00 EDT 2023

Transcriptome sequencing identifies prognostic genes involved in gastric adenocarcinoma.

Molecular and cellular biochemistry

Li M,Bai M,Wu Y,Yang S,Zheng L,Sun L,Yu C,Huang Y

Fri Dec 01 00:00:00 EST 2023

Antiovarian cancer mechanism of esculetin: inducing G0/G1 arrest and apoptosis via JAK2/STAT3 signalling pathway.

The Journal of pharmacy and pharmacology

Yin W,Fu X,Chang W,Han L,Meng J,Cao A,Ren X,Fan Z,Zhou S

Tue Jan 31 00:00:00 EST 2023

Analysis of regulating activities of 5'-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo.

Frontiers in pharmacology

Wang X,Luo X,Gan X,Chen C,Yang Z,Wen J,Fang W,Huang H,Gao C,Zhou X,Feng X,Liu Y

Tue Aug 30 00:00:00 EDT 2022

LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway.

Cellular oncology (Dordrecht)

Yue H,Wu K,Liu K,Gou L,Huang A,Tang H

Wed Jun 01 00:00:00 EDT 2022

The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

Ecotoxicology and environmental safety

Wu J,Yang C,Yang M,Liang Z,Wu Y,Kong X,Fan H,Wang S,Ning C,Xiao W,Jin Y,Yi J,Yuan Z

Wed Jun 01 00:00:00 EDT 2022

LncRNA Airn alleviates diabetic cardiac fibrosis by inhibiting activation of cardiac fibroblasts via a m6A-IMP2-p53 axis.

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Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

ACS pharmacology & translational science

Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

Fri Jul 08 00:00:00 EDT 2022

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

ACS pharmacology & translational science

Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

Fri Jul 08 00:00:00 EDT 2022

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

ACS pharmacology & translational science

Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

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Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

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Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

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RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway.

International journal of molecular sciences

Xu J,Shi P,Xia F,Zhao X,Chen J,Geng R,Cui H,Yang L

Mon Oct 10 00:00:00 EDT 2022

Membrane-associated RING-CH protein (MARCH8) is a novel glycolysis repressor targeted by miR-32 in colorectal cancer.

Journal of translational medicine

Wang Z,Wang MM,Geng Y,Ye CY,Zang YS

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MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation.

Oxidative medicine and cellular longevity

Qi C,Min P,Wang Q,Wang Y,Song Y,Zhang Y,Bibi M,Du J

Mon Feb 07 00:00:00 EST 2022

LHPP-Mediated Histidine Dephosphorylation Suppresses the Self-Renewal of Mouse Embryonic Stem Cells.

Frontiers in cell and developmental biology

Xia RM,Yao DB,Cai XM,Xu XQ

Sat Apr 03 00:00:00 EDT 2021

Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis.

Molecular and cellular endocrinology

He ZY,Huang MT,Cui X,Zhou ST,Wu Y,Zhang PH,Zhou J

Thu Jul 15 00:00:00 EDT 2021

PSMD7 downregulation suppresses lung cancer progression by regulating the p53 pathway.

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The novel LSD1 inhibitor ZY0511 suppresses diffuse large B-cell lymphoma proliferation by inducing apoptosis and autophagy.

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Liu H,Wei J,Sang N,Zhong X,Zhou X,Yang X,Zhang J,Zuo Z,Zhou Y,Yang S,Du J,Zhao Y

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Bioinformatics

Protein Aliases: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34/cdk4; PSK-J3; serine/threonine kinase

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Gene Aliases: CDK4; CMM3; Crk3; PSK-J3

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UniProt ID: (Human) P11802, (Rat) P35426, (Mouse) P30285

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Entrez Gene ID: (Human) 1019, (Rat) 94201, (Mouse) 12567

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Function(s)

cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding cyclin-dependent protein serine/threonine kinase regulator activity cyclin binding protein complex binding protein kinase activity kinase activity nucleotide binding protein serine/threonine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle lens development in camera-type eye protein phosphorylation signal transduction circadian rhythm positive regulation of cell proliferation response to toxic substance response to lead ion regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle organ regeneration response to testosterone response to drug positive regulation of apoptotic process positive regulation of translation positive regulation of cell cycle positive regulation of cell size regulation of protein kinase activity positive regulation of fibroblast proliferation cell division response to hyperoxia negative regulation of cell cycle arrest response to organic substance regulation of cell proliferation regulation of cell cycle cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK4 Monoclonal Antibody (1G2C12)

CDK4 Antibody (66950-1-IG) in IHC (P)

Product Details

66950-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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