Invitrogen

CDK4 Monoclonal Antibody (DCS-35), Biotin

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CDK4 Antibody (MA5-12981) in WB

Western blot of Cdk4 using Cdk4 Monoclonal Antibody (Product # MA5-12981) on MAD109 Cells.

CDK4 Antibody in Immunoprecipitation (IP)

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Product Details

MA5-12981

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DCS-35

Immunogen

Purified recombinant cdk4 protein

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980320

Product Specific Information

MA5-12981 targets Cdk4 in IF, IP, and WB applications and shows reactivity with Human, mouse, Porcine, and Rat samples.

The MA5-12981 immunogen is purified recombinant cdk4 protein.

Target Information

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells.

Biochemical and biophysical research communications

Naoe H,Chiyoda T,Ishizawa J,Masuda K,Saya H,Kuninaka S

MA5-12981 was used in western blot to study the role of Cdh1 in regulating G2/M transition during placental differentiation

Fri Jan 11 00:00:00 EST 2013

Menthol inhibits the proliferation and motility of prostate cancer DU145 cells.

Pathology oncology research : POR

Wang Y,Wang X,Yang Z,Zhu G,Chen D,Meng Z

MA5-12981 was used in western blot to study the effects of TRPM8 activation on prostate cancer cell proliferation and migration

Mon Oct 01 00:00:00 EDT 2012

Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.

Molecular and cellular biology

James MK,Ray A,Leznova D,Blain SW

MA5-12981 was used in immunoprecipitation to study if the growth state-dependent tyrosine phosphorylation of p27Kip1 determines whether or not it inhibits cdk4

Tue Jan 01 00:00:00 EST 2008

Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation.

Molecular biology of the cell

Rocha AS,Paternot S,Coulonval K,Dumont JE,Soares P,Roger PP

MA5-12981 was used in immunoprecipitation to study the role of CDK4 phosphorylation in the mechanism by which cAMP inhibits thyroid carcinoma cell proliferation

Sat Nov 01 00:00:00 EDT 2008

Regulation of keratinocyte proliferation in rats with deep, partial-thickness scald: modulation of cyclin D1-cyclin-dependent kinase 4 and histone H1 kinase activity of M-phase promoting factor.

The Journal of surgical research

Xie T,Niu Y,Ge K,Lu S

MA5-12981 was used in western blot to study the mechanism of regulation of keratinocyte proliferation in rats with deep partial-thickness scald

Sun Jun 01 00:00:00 EDT 2008

The LxCxE pRb interaction domain of cyclin D1 is dispensable for murine development.

Cancer research

Landis MW,Brown NE,Baker GL,Shifrin A,Das M,Geng Y,Sicinski P,Hinds PW

MA5-12981 was used in western blot to study whether the LxCxE domain of cyclin D1 which interacts with the retinoblastoma protein is required for normal murine development

Wed Aug 15 00:00:00 EDT 2007

Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.

Cancer research

Hudson TS,Hartle DK,Hursting SD,Nunez NP,Wang TT,Young HA,Arany P,Green JE

MA5-12981 was used in western blot to study the distinct mechanisms by which muscadine grape skin extract and resveratrol inhibit prostate cancer cell growth

Sat Sep 01 00:00:00 EDT 2007

Critical requirement for cell cycle inhibitors in sustaining nonproliferative states.

The Journal of cell biology

Pajalunga D,Mazzola A,Salzano AM,Biferi MG,De Luca G,Crescenzi M

MA5-12981 was used in immunoprecipitation to examine the role of cell cycle inhibitors in cell growth arrest

Mon Mar 12 00:00:00 EDT 2007

Requirement for CDK4 kinase function in breast cancer.

Cancer cell

Yu Q,Sicinska E,Geng Y,Ahnström M,Zagozdzon A,Kong Y,Gardner H,Kiyokawa H,Harris LN,Stål O,Sicinski P

MA5-12981 was used in immunoprecipitation to study the importance of cyclin D1-dependent Cdk4 kinase activity in mammary tumorigenesis

Sun Jan 01 00:00:00 EST 2006

The role of p28GANK in rat oval cells activation and proliferation.

Liver international : official journal of the International Association for the Study of the Liver

Shan YF,Zhou WP,Fu XY,Yan HX,Yang W,Liu SQ,Cao HF,Kang B,Wu MC,Wang HY

MA5-12981 was used in western blot to investigate the importance of p28GANK in the activation and proliferation of rat oval cells

Wed Mar 01 00:00:00 EST 2006

In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202.

Clinical cancer research : an official journal of the American Association for Cancer Research

Raynaud FI,Whittaker SR,Fischer PM,McClue S,Walton MI,Barrie SE,Garrett MD,Rogers P,Clarke SJ,Kelland LR,Valenti M,Brunton L,Eccles S,Lane DP,Workman P

MA5-12981 was used in western blot to study the pharmacokinetics and pharmacodynamics of three tri-substituted aminopurine Cdk inhibitors in a model of colon carcinoma

Fri Jul 01 00:00:00 EDT 2005

p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2.

The Journal of cell biology

Rodier G,Makris C,Coulombe P,Scime A,Nakayama K,Nakayama KI,Meloche S

MA5-12981 was used in western blot to study the role of Skp2 in the mechanism by which p107 inhibits G1/S phase progression

Mon Jan 03 00:00:00 EST 2005

p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells.

The Journal of biological chemistry

Muñoz-Alonso MJ,Acosta JC,Richard C,Delgado MD,Sedivy J,León J

MA5-12981 was used in immunoprecipitation to study the role of p21Cip1 and p27Kip1 in the cell cycle and differentiation programs of myeloid leukemia cells

Fri May 06 00:00:00 EDT 2005

p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.

International journal of cancer

Jayasurya R,Francis G,Kannan S,Lekshminarayanan K,Nalinakumari KR,Abraham T,Abraham EK,Nair MK

MA5-12981 was used in immunohistochemistry to study the molecular determinants of the response to radiotherapy in oral carcinoma

Sat May 01 00:00:00 EDT 2004

Regulation and role of p21 and p27 cyclin-dependent kinase inhibitors during hepatocyte differentiation and growth.

American journal of physiology. Gastrointestinal and liver physiology

Ilyin GP,Glaise D,Gilot D,Baffet G,Guguen-Guillouzo C

MA5-12981 was used in western blot to study the role of the p21 and p27 cyclin-dependent kinase inhibitors in hepatocyte development and proliferation

Tue Jul 01 00:00:00 EDT 2003

Interaction of Hsp90 with the nascent form of the mutant epidermal growth factor receptor EGFRvIII.

The Journal of biological chemistry

Lavictoire SJ,Parolin DA,Klimowicz AC,Kelly JF,Lorimer IA

MA5-12981 was used in western blot to study the interaction of nascent EGFRvIII with Hsp90 and its importance for maintaining EGFRvIII expression

Fri Feb 14 00:00:00 EST 2003

Autoimmune responses to proliferating cell nuclear antigen multiprotein complexes involved in cell proliferation are strongly associated with their structure and biologic function in patients with systemic lupus erythematosus.

Arthritis and rheumatism

Kogure T,Takasaki Y,Takeuchi K,Yamada H,Nawata M,Ikeda K,Matsushita M,Matsudaira R,Kaneda K,Terasawa K,Hashimoto H

MA5-12981 was used in ELISA to study the mechanism of autoimmune responses to PCNA and its associated proteins in systemic lupus erythematosus

Fri Nov 01 00:00:00 EST 2002

Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.

Molecular and cellular biology

Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H

MA5-12981 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown

Fri Oct 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34/cdk4; PSK-J3; serine/threonine kinase

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Gene Aliases: CDK4; CMM3; Crk3; PSK-J3

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UniProt ID: (Pig) P79432, (Human) P11802, (Rat) P35426, (Mouse) P30285

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Entrez Gene ID: (Pig) 100144492, (Human) 1019, (Rat) 94201, (Mouse) 12567

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Function(s)

ATP binding cyclin binding cyclin-dependent protein serine/threonine kinase activity protein binding cyclin-dependent protein serine/threonine kinase regulator activity protein complex binding protein kinase activity kinase activity nucleotide binding protein serine/threonine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle signal transduction regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle cellular response to insulin stimulus regulation of multicellular organism growth response to drug regulation of insulin receptor signaling pathway regulation of lipid biosynthetic process positive regulation of fibroblast proliferation regulation of lipid catabolic process cell division adipose tissue development negative regulation of cell cycle arrest cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to phorbol 13-acetate 12-myristate cellular response to ionomycin lens development in camera-type eye protein phosphorylation circadian rhythm positive regulation of cell proliferation response to toxic substance response to lead ion organ regeneration response to testosterone positive regulation of apoptotic process positive regulation of translation positive regulation of cell cycle positive regulation of cell size regulation of protein kinase activity response to hyperoxia response to organic substance regulation of cell proliferation regulation of cell cycle cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK4 Monoclonal Antibody (DCS-35), Biotin

CDK4 Antibody (MA5-12981) in WB

Product Details

MA5-12981

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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