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Invitrogen
CDK6 Recombinant Rabbit Monoclonal Antibody (ARC0224)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
CDK6 Antibody (MA5-35046) in ICC/IF
Immunofluorescence analysis of CDK6 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDK6 Recombinant Rabbit Monoclonal Antibody (ARC0224) (Product # MA5-35046) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More
Product Details
MA5-35046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0224
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 227-326 of human CDK6 (Q00534)
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848951
Product Specific Information
Immunogen sequence: QLGKILDVIG LPGEEDWPRD VALPRQAFHS KSAQPIEKFV TDIDELGKDL LLKCLTFNPA KRISAYSALS HPYFQDLERC KENLDSHLPP SQNTSELNTA
Target Information
CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 6; Cyclin-dependent kinase 6; MGC59692; Serine/threonine-protein kinase PLSTIRE
Gene Aliases: CDK6; CDKN6; MCPH12; PLSTIRE
UniProt ID: (Human) Q00534
Entrez Gene ID: (Human) 1021, (Rat) 114483
G1/S transition of mitotic cell cycle
positive regulation of cell-matrix adhesion
type B pancreatic cell development
protein phosphorylation
cell cycle arrest
Notch signaling pathway
negative regulation of cell proliferation
response to virus
regulation of gene expression
positive regulation of gene expression
astrocyte development
dentate gyrus development
lateral ventricle development
T cell differentiation in thymus
gliogenesis
cell dedifferentiation
negative regulation of cell differentiation
negative regulation of myeloid cell differentiation
regulation of erythrocyte differentiation
negative regulation of monocyte differentiation
negative regulation of osteoblast differentiation
negative regulation of cell cycle
positive regulation of fibroblast proliferation
generation of neurons
negative regulation of epithelial cell proliferation
cell division
hematopoietic stem cell differentiation
cellular response to phorbol 13-acetate 12-myristate
regulation of cell motility
negative regulation of cellular senescence
hematopoietic progenitor cell differentiation
phosphorylation
hemopoiesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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