Proteintech

CEBPA Polyclonal Antibody

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CEBPA Antibody (18311-1-AP) in WB

Human liver tissue were subjected to SDS PAGE followed by western blot with 18311-1-AP (CEBPA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

CEBPA Antibody in Immunoprecipitation (IP)

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Product Details

18311-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 43 publications 43 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients.

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miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.

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Pleiotropic effects of cavin-1 deficiency on lipid metabolism.

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Ding SY,Lee MJ,Summer R,Liu L,Fried SK,Pilch PF

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Pleiotropic effects of cavin-1 deficiency on lipid metabolism.

The Journal of biological chemistry

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Bioinformatics

Protein Aliases: C EBP; C/EBP alpha; CAAT/enhancer-binding protein DNA-binding protein; CAAT/enhancer-binding protein, DNA-binding protein; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer binding protein, alpha; CCAAT/enhancer-binding protein alpha; OTTHUMP00000220269

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Gene Aliases: C/EBP-alpha; C/ebpalpha; CBF-A; CEBP; CEBPA; DBPCEP

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UniProt ID: (Human) P49715, (Mouse) P53566, (Rat) P05554

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Entrez Gene ID: (Human) 1050, (Mouse) 12606, (Rat) 24252

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding kinase binding protein homodimerization activity transcription regulatory region DNA binding RNA polymerase I regulatory region DNA binding protein domain specific binding protein complex binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity HMG box domain binding basic leucine zipper transcription factor

Process(es)

urea cycle negative regulation of transcription from RNA polymerase II promoter liver development embryonic placenta development generation of precursor metabolites and energy transcription, DNA-templated transcription from RNA polymerase II promoter mitochondrion organization Notch signaling pathway cholesterol metabolic process negative regulation of cell proliferation viral process cytokine-mediated signaling pathway myeloid cell differentiation macrophage differentiation lung development granulocyte differentiation positive regulation of proteasomal ubiquitin-dependent protein catabolic process glucose homeostasis fat cell differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase III promoter cell maturation inner ear development white fat cell differentiation brown fat cell differentiation lipid homeostasis cellular response to lithium ion cellular response to organic cyclic compound regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase I promoter multicellular organism development regulation of cell proliferation negative regulation of cell cycle positive regulation of transcription, DNA-templated acute-phase response response to nutrient cell differentiation organ regeneration response to vitamin B2 response to glucocorticoid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CEBPA Polyclonal Antibody

CEBPA Antibody (18311-1-AP) in WB

Product Details

18311-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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