Invitrogen

CENPA Monoclonal Antibody (3-19)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 References

View all (23) CENPA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Advanced Verification (1)

CENPA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

CENPA Antibody (MA1-20832) in ICC/IF

Immunofluorescence analysis of CENPA was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766... View More

CENPA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CENPA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HT-29 cells were transfected with CENPA siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CENPA Monoclonal Antibody (3-19) (Product # MA1-20832). {KD}

Product Details

MA1-20832

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-dependent

CUT&RUN (C&R)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3-19

Immunogen

Synthetic peptide corresponding to residues P(3) R R R S R K P E A P R R R S P S(19) of human CENPA protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2078763

Product Specific Information

Recommended positive controls: , BE cells.

This antibody does not cross react with mouse CENPA proteins.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer.

Molecular biology of the cell

Rodrigues A,MacQuarrie KL,Freeman E,Lin A,Willis AB,Xu Z,Alvarez AA,Ma Y,White BEP,Foltz DR,Huang S

MA1-20832 was used in Immunocytochemistry-immunofluorescence to suggest active roles of nucleolar structure in centromere function and genome organization critical for cellular function in both normal development and cancer.

Sat Apr 01 00:00:00 EDT 2023

The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.

Journal of cell science

Shrestha RL,Balachandra V,Kim JH,Rossi A,Vadlamani P,Sethi SC,Ozbun L,Lin S,Cheng KC,Chari R,Karpova TS,Pegoraro G,Foltz DR,Caplen NJ,Basrai MA

MA1-20832 was used in CUT&RUN to identify regulators of CENP-A localization and defined a role for CHAF1B in preventing DAXX-dependent CENP-A mislocalization and CIN.

Mon May 15 00:00:00 EDT 2023

Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.

Nature communications

Smurova K,Damizia M,Irene C,Stancari S,Berto G,Perticari G,Iacovella MG,D'Ambrosio I,Giubettini M,Philippe R,Baggio C,Callegaro E,Casagranda A,Corsini A,Polese VG,Ricci A,Dassi E,De Wulf P

MA1-20832 was used in Western Blot to suggest that CEN regulation by Rio1/RioK1 is evolutionary conserved.

Thu Jun 01 00:00:00 EDT 2023

Exploration of a Novel Prognostic Nomogram and Diagnostic Biomarkers Based on the Activity Variations of Hallmark Gene Sets in Hepatocellular Carcinoma.

Frontiers in oncology

Zhong X,Yu X,Chang H

MA1-20832 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Neutralization, Western Blot to study novel biomarkers based on gene set variation analysis (GSVA) in hepatocellular carcinoma.

Sat Apr 02 00:00:00 EDT 2022

Holliday junction recognition protein as a prognostic biomarker and therapeutic target for oral cancer.

International journal of oncology

Tsevegjav B,Takano A,Zhu M,Yoshitake Y,Shinohara M,Daigo Y

MA1-20832 was used in Immunoprecipitation to indicate that Holliday junction recognition protein (HJURP) is a potential prognostic biomarker, and targeting HJURP and its molecular pathway presents a new strategy for the development of treatments against oral cancer.

Tue Mar 01 00:00:00 EST 2022

Inheritance of CENP-A Nucleosomes during DNA Replication Requires HJURP.

Developmental cell

Zasadzińska E,Huang J,Bailey AO,Guo LY,Lee NS,Srivastava S,Wong KA,French BT,Black BE,Foltz DR

MA1-20832 was used in Immunocytochemistry to identify proteins transiently associated with CENP-A during DNA replication.

Mon Nov 05 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A

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Gene Aliases: CenH3; CENP-A; CENPA; ggCENP-A

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UniProt ID: (Human) P49450, (Chicken) Q6XXM1

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Entrez Gene ID: (Human) 1058, (Chicken) 100113359

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Function(s)

DNA binding chromatin binding protein binding protein heterodimerization activity chromatin/chromatin-binding, or -regulatory protein histone

Process(es)

establishment of mitotic spindle orientation sister chromatid cohesion viral process CENP-A containing nucleosome assembly kinetochore assembly protein localization to chromosome, centromeric region mitotic cytokinesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CENPA Monoclonal Antibody (3-19)

CENPA Antibody (MA1-20832) in ICC/IF

Product Details

MA1-20832

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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