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CENPJ Antibody (PA5-100718) in ICC/IF
Immunocytochemistry analysis of CENPJ in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CENPJ polyclonal antibody (Product # PA5-100718) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CENPJ(Accession Q9HC77), corresponding to amino acid residues F1265-E1315.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850222
Product Specific Information
Antibody detects endogenous levels of total CENPJ.
Target Information
This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP-J; Centromere protein J; Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1; LYST-interacting protein LIP1; LYST-interacting protein LIP7; microcephaly
Gene Aliases: 4932437H03Rik; BM032; CENP-J; CENPJ; CPAP; Gm81; LAP; LIP1; MCPH6; Sas-4; Sas4; SASS4; SCKL4
UniProt ID: (Human) Q9HC77, (Mouse) Q569L8
Entrez Gene ID: (Human) 55835, (Mouse) 219103
G2/M transition of mitotic cell cycle
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
microtubule nucleation
centriole replication
regulation of centriole replication
microtubule polymerization
cell division
centriole elongation
astral microtubule nucleation
positive regulation of JAK-STAT cascade
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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