Proteintech

CHMP2B Polyclonal Antibody

View all (21) CHMP2B antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (18)

CHMP2B Antibody in Immunocytochemistry (ICC/IF)

CHMP2B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

CHMP2B Antibody (12527-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CHMP2B Antibody in Immunocytochemistry (ICC/IF)

CHMP2B Antibody in Immunocytochemistry (ICC/IF)

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

CHMP2B Antibody in Western Blot (WB)

Product Image Gallery

CHMP2B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CHMP2B Antibody in Immunocytochemistry (ICC/IF) — IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman.

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 12527-1-AP) CHMP2B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue slide using (Product # 12527-1-AP) CHMP2B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens).

CHMP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens).

CHMP2B Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — Human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CHMP2B Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

12527-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHMP2B Fusion Protein Ag3222 (1-213 aa encoded by BC001553)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MASLFKKKT VDDVIKEQNR ELRGTQRAII RDRAALEKQE KQLELEIKKM AKIGNKEACK VLAKQLVHLR KQKTRTFAVS SKVTSMSTQT KVMNSQMKMA GAMSTTAKTM QAVNKKMDPQ KTLQTMQNFQ KENMKMEMTE EMINDTLDDI FDGSDDEEES QDIVNQVLDE IGIEISGKMA KAPSAARSLP SASTSKATIS DEEIERQLKA LGVD (1-213 aa encoded by BC001553)

Target Information

CHMP2B, also known as CHMP2.5 or vacuolar protein-sorting-associated protein 2-2, is a 213 amino acid cytosolic protein. Widely expressed in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes, CHMP2B associates directly with CHMP2A and vps4 for the disassembly of the ESCRT-III complex. Defects in the gene encoding CHMP2B have been shown to cause chromosome 3-linked frontotemporal dementia (FTD3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Charged multivesicular body protein 2B ameliorates biliary injury in the liver from donation after cardiac death rats via autophagy with air-oxygenated normothermic machine perfusion.

Biochimica et biophysica acta. Molecular basis of disease

Bai Y,Shi JH,Liu Q,Yang DJ,Yan ZP,Zhang JK,Tang HW,Guo WZ,Jin Y,Zhang SJ

Thu Jun 01 00:00:00 EDT 2023

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 EST 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 EST 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 EST 2022

SIRT6 Protects Against Myocardial Ischemia-Reperfusion Injury by Attenuating Aging-Related CHMP2B Accumulation.

Journal of cardiovascular translational research

Li X,Liu L,Jiang W,Liu M,Wang Y,Ma H,Mu N,Wang H

Mon Aug 01 00:00:00 EDT 2022

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 EDT 2020

Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread.

Journal of virology

Streck NT,Zhao Y,Sundstrom JM,Buchkovich NJ

Thu Jul 30 00:00:00 EDT 2020

Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.

Metabolic brain disease

Gao W,Wang W,Liu G,Zhang J,Yang J,Deng Z

Fri Feb 01 00:00:00 EST 2019

Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.

Metabolic brain disease

Gao W,Wang W,Liu G,Zhang J,Yang J,Deng Z

Fri Feb 01 00:00:00 EST 2019

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 EST 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 EST 2018

Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.

International journal for parasitology. Drugs and drug resistance

Ballesteros C,Geary JF,Mackenzie CD,Geary TG

Wed Aug 01 00:00:00 EDT 2018

Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.

International journal for parasitology. Drugs and drug resistance

Ballesteros C,Geary JF,Mackenzie CD,Geary TG

Wed Aug 01 00:00:00 EDT 2018

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Cell death & disease

Gao K,Zhang Y,Shi Q,Zhang J,Zhang L,Sun H,Jiao D,Zhao X,Tao H,Wei Y,Wang Y,Saiyin H,Zhao SM,Li Y,Zhang P,Wang C

Tue May 01 00:00:00 EDT 2018

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Cell death & disease

Gao K,Zhang Y,Shi Q,Zhang J,Zhang L,Sun H,Jiao D,Zhao X,Tao H,Wei Y,Wang Y,Saiyin H,Zhao SM,Li Y,Zhang P,Wang C

Tue May 01 00:00:00 EDT 2018

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Oncotarget

Huo Y,Zheng Z,Chen Y,Wang Q,Zhang Z,Deng H

Tue Jul 19 00:00:00 EDT 2016

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Oncotarget

Huo Y,Zheng Z,Chen Y,Wang Q,Zhang Z,Deng H

Tue Jul 19 00:00:00 EDT 2016

Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells.

Journal of cellular biochemistry

Cheng CM,Wang D,Cao X,Luo QQ,Lu YP,Zhu L

Tue Sep 01 00:00:00 EDT 2015

Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells.

Journal of cellular biochemistry

Cheng CM,Wang D,Cao X,Luo QQ,Lu YP,Zhu L

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Charged multivesicular body protein 2b; CHMP family; CHMP2.5; CHMP2b; chromatin modifying protein 2B; Chromatin-modifying protein 2b; hVps2-2; Vacuolar protein sorting-associated protein 2-2; vacuolar protein-sorting-associated protein 2-2; VPS2 homolog B; Vps2-2

View more View less

Gene Aliases: 1190006E07Rik; ALS17; CGI-84; CHMP2.5; CHMP2B; DMT1; RGD1306781; VPS2-2; VPS2B

View more View less

UniProt ID: (Human) Q9UQN3, (Mouse) Q8BJF9

View more View less

Entrez Gene ID: (Human) 25978, (Mouse) 68942, (Rat) 363720

View more View less

protein binding protein domain specific binding cadherin binding involved in cell-cell adhesion molecular_function membrane traffic protein

Process(es)

cell separation after cytokinesis autophagy nucleus organization endosome organization mitotic metaphase plate congression regulation of centrosome duplication protein transport endosomal transport viral life cycle multivesicular body assembly viral budding via host ESCRT complex cognition neuron cellular homeostasis multivesicular body sorting pathway cell-cell adhesion regulation of mitotic spindle assembly positive regulation of viral release from host cell ESCRT III complex disassembly transport vacuolar transport biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support