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CHMP4B Antibody (PA5-100092) in ICC/IF
Immunofluorescent analysis of CHMP4B in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CHMP4B polyclonal antibody (Product # PA5-100092) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-100092
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHMP4B(Accession Q9H444), corresponding to amino acid residues M1-E50.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815622
Product Specific Information
Antibody detects endogenous levels of total CHMP4B.
Target Information
CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Charged multivesicular body protein 4b; CHMP4b; chromatin modifying protein 4B; Chromatin-modifying protein 4b; hSnf7-2; hVps32-2; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2; Vps32-2
Gene Aliases: 2010012F05Rik; C20orf178; C76846; CHMP4A; CHMP4B; CTPP3; CTRCT31; dJ553F4.4; RGD1309846; RGD1565889; SHAX1; SNF7; SNF7-2; Vps32-2; VPS32B
UniProt ID: (Human) Q9H444, (Mouse) Q9D8B3
Entrez Gene ID: (Human) 128866, (Mouse) 75608, (Rat) 100359642
mitotic cytokinesis
cell separation after cytokinesis
posttranslational protein targeting to membrane
autophagy
nucleus organization
mitotic metaphase plate congression
exit from mitosis
regulation of centrosome duplication
endosomal transport
viral life cycle
nuclear envelope reassembly
multivesicular body assembly
maintenance of lens transparency
viral budding via host ESCRT complex
viral budding
regulation of viral process
protein homooligomerization
negative regulation of cell death
membrane fission
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
cell-cell adhesion
negative regulation of neuron death
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
negative regulation of autophagosome assembly
transport
vacuolar transport
protein transport
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