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Proteintech
cyclin B1 Monoclonal Antibody (1F3G11), CoraLite® Plus 647
FIGURE: 1 / 1
cyclin B1 Antibody (CL647-67686) in Flow
1X10^6 HeLa cells were intracellularly stained with 0.2 µg CoraLite® Plus 647 Anti-Human cyclin B1 (CL647-67686, Clone:1F3G11) (red), or 0.2 µg isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Product Details
CL647-67686
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F3G11
Immunogen
Recombinant protein
Conjugate
Excitation/Emission Max
654/674 nm
Form
Liquid
Purification
Protein G
Storage buffer
PBS with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, store in dark
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023
Gene Aliases: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1
UniProt ID: (Human) P14635, (Rat) P30277, (Mouse) P24860
Entrez Gene ID: (Human) 891, (Rat) 25203, (Mouse) 268697
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
negative regulation of protein phosphorylation
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic spindle organization
mitotic nuclear envelope disassembly
mitotic metaphase plate congression
spermatogenesis
response to mechanical stimulus
negative regulation of gene expression
anaphase-promoting complex-dependent catabolic process
positive regulation of mRNA 3'-end processing
positive regulation of histone phosphorylation
tissue regeneration
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of cell cycle
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to organic cyclic compound
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
histone H3-S10 phosphorylation involved in chromosome condensation
protein phosphorylation
cell cycle
mitotic nuclear division
positive regulation of cell cycle
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