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CMKLR1 Antibody (PA5-90310) in WB
Western blot analysis of CMKLR1 in extracts of various cell lines. Samples were incubated with CMKLR1 Polyclonal antibody (Product # PA5-90310) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90310
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CMKLR1 (NP_0011358151)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806017
Product Specific Information
Immunogen sequence: NRLAKTKKPF KIIVTIIITF FLCWCPYHTL NLLELHHTAM PGSVFSLGLP LATALAIANS CMNPILYVFM GQDFKKFKVA LFSRLVNALS EDTGHSSYPS H; Positive Samples: LO2, Mouse brain; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
Chemokine-like receptor 1 (CMKLR1) is a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 positively regulates adipogenesis and adipocyte metabolism and acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chemerin receptor; Chemerin-like receptor 1; chemokine receptor-like 1; Chemokine-like receptor 1; G-protein coupled receptor 27; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; resolvin E1 receptor
Gene Aliases: CHEMERINR; CHEMR23; CMKLR1; DEZ; Gpcr27; mcmklr1; RVER1
UniProt ID: (Human) Q99788, (Mouse) P97468
Entrez Gene ID: (Human) 1240, (Mouse) 14747
skeletal system development
chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of macrophage chemotaxis
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-12 production
positive regulation of fat cell differentiation
regulation of calcium-mediated signaling
chemokine-mediated signaling pathway
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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