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Invitrogen
CMKLR1 Recombinant Rabbit Monoclonal Antibody (7H10L22)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
CMKLR1 Antibody (703553) in ICC/IF
Knockdown of CMKLR1 was achieved by transfecting Hep G2 cells with specific siRNA (Silencer® select Product # s3226). Immunofluorescence analysis was performed on Hep G2 cells (untransfected, panel a-d), transfected with CMKLR1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-CMKLR1 Recombinant Rabbit Monoclonal Antibody (Product # 703553, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Product Details
703553
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H10L22
Immunogen
Peptides corresponding to human CMKLR1 [1) aa11-aa24 2) aa360-aa373]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784587
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Dog.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Chemokine-like receptor 1 (CMKLR1) is a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 positively regulates adipogenesis and adipocyte metabolism and acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chemerin receptor; Chemerin-like receptor 1; chemokine receptor-like 1; Chemokine-like receptor 1; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; resolvin E1 receptor
Gene Aliases: CHEMERINR; CHEMR23; CMKLR1; DEZ; RVER1
UniProt ID: (Human) Q99788
Entrez Gene ID: (Human) 1240
skeletal system development
chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of macrophage chemotaxis
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-12 production
positive regulation of fat cell differentiation
regulation of calcium-mediated signaling
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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