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Invitrogen
CMKLR1 Chimeric Recombinant Rabbit Monoclonal Antibody (BZ194)
FIGURE: 1 / 1
CMKLR1 Chimeric Antibody (MA5-48145) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CMKLR1 in RAW264.7 cells using CMKLR1 Chimeric Monoclonal Antibody (Product # MA5-48145). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CMKLR1 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from ... View More
Product Details
MA5-48145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BZ194
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide (aa 8-32) /KLH conjugate.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074588
Product Specific Information
Specificity: This antibody is specific for murine serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mCMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.
Preparation: This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Target Information
Chemokine-like receptor 1 (CMKLR1) is a receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 positively regulates adipogenesis and adipocyte metabolism and acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chemerin receptor; Chemerin-like receptor 1; chemokine receptor-like 1; Chemokine-like receptor 1; G-protein coupled receptor 27; G-protein coupled receptor DEZ
Gene Aliases: ChemR23; Cmklr1; DEZ; Gpcr27; mcmklr1
UniProt ID: (Mouse) P97468
Entrez Gene ID: (Mouse) 14747
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of macrophage chemotaxis
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-12 production
positive regulation of fat cell differentiation
regulation of calcium-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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