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CNGB1 Antibody (PA5-101543) in ICC/IF
Immunofluorescent analysis of CNGB1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CNGB1 polyclonal antibody (Product # PA5-101543) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101543
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CNGB1(Accession Q14028), corresponding to amino acid residues V917-D967.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850977
Product Specific Information
Antibody detects endogenous levels of total CNGB1.
Target Information
Glutamic acid rich protein (GARP) is a soluble protein localized to the outer segments of the rod photoreceptor. GARPs are characterized by six repeated motifs of: V V E K K N/E E. These repeat motifs help form an alpha-helix structure. The function(s) of GARP have not been fully characterized, but studies have suggested that GARP helps limit cGMP turnover by inhibiting phosphodiesterase activity. To date, there have been two splice variants characterized: GARP1 (full-length GARP) and GARP2 (truncated GARP). GARP also interacts with part of the larger cytoplasmic domain of the beta-subunit of the cyclic GMP-gated channel found in the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cng channel; CNG channel 4; CNG channel beta-1; CNG-4; Cyclic nucleotide-gated cation channel 4; cyclic nucleotide-gated cation channel beta-1; Cyclic nucleotide-gated cation channel gamma; Cyclic nucleotide-gated cation channel modulatory subunit; cyclic nucleotide-gated channel beta subunit 1; Cyclic nucleotide-gated channel beta-1; GARP; Glutamic acid-rich protein; glutamic-acid-rich protein
Gene Aliases: CNCG2; CNCG3L; CNCG4; CNG4; CNGB1; CNGB1B; GAR1; GARP; GARP2; Gm1959; RCNC2; RCNCb; RCNCbeta; RP45
UniProt ID: (Human) Q14028, (Mouse) E1AZ71
Entrez Gene ID: (Human) 1258, (Rat) 83686, (Mouse) 333329
intracellular cAMP activated cation channel activity
intracellular cGMP activated cation channel activity
voltage-gated potassium channel activity
protein binding
ligand-gated ion channel activity
cAMP binding
cGMP binding
intracellular cyclic nucleotide activated cation channel activity
cyclic nucleotide-gated ion channel activity
ligand-gated ion channel
retina homeostasis
transport
cation transport
visual perception
sensory perception of smell
rhodopsin mediated signaling pathway
regulation of rhodopsin mediated signaling pathway
protein localization to organelle
photoreceptor cell outer segment organization
regulation of membrane potential
photoreceptor cell maintenance
detection of light stimulus involved in visual perception
potassium ion transmembrane transport
phototransduction
phototransduction, visible light
ion transmembrane transport
protein heterotetramerization
regulation of cytosolic calcium ion concentration
regulation of ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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