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CNOT8 Antibody (PA5-13451) in ICC/IF
Immunocytochemistry analysis of CNOT8 in HeLa cells. Samples were incubated with CNOT8 polyclonal antibody (Product # PA5-13451) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). CNOT8... View More
Product Details
PA5-13451
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2082471
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CALIF or POP2, is a 292 amino acid protein that localizes to both the nucleus and the cytoplasm and functions as part of the CCR-NOT complex. Expressed ubiquitously, CNOT8 plays a role in transcriptional regulation for a diverse set of processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAF1-like protein; Caf1b; CAF2; CALIFp; CCR4-associated factor 8; CCR4-NOT transcription complex subunit 8; CCR4-NOT transcription complex, subunit 8; h CAF1-like protein; PGK promoter directed over production
Gene Aliases: CAF1; Caf1b; CALIF; CNOT8; hCAF1; POP2
UniProt ID: (Human) Q9UFF9
Entrez Gene ID: (Human) 9337
nuclear-transcribed mRNA poly(A) tail shortening
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
positive regulation of cell proliferation
negative regulation of cell proliferation
gene silencing by miRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
positive regulation of mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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