Invitrogen

COL1A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

13 References

View all (11) COL1A1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

COL1A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

COL1A1 Antibody (PA5-29569) in ICC/IF

Immunofluorescence analysis of COL1A1 was performed using 70% confluent log phase SK-N-SH and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL1A1 Polyclonal Antibody (Product # PA5-29569) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More

COL1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COL1A1 Antibody in Immunoprecipitation (IP)

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Product Details

PA5-29569

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 219 and 478 of Human COL1A1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547045

Product Specific Information

Recommended positive controls: SK-N-SH, SK-N-AS, NIH3T3.

Predicted reactivity: Mouse (96%), Rat (94%), Xenopus laevis (83%), Dog (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is the pro-alpha1 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Kaempferide Prevents Photoaging of Ultraviolet-B Irradiated NIH-3T3 Cells and Mouse Skin via Regulating the Reactive Oxygen Species-Mediated Signalings.

Antioxidants (Basel, Switzerland)

Choi JK,Kwon OY,Lee SH

Published figure using COL1A1 polyclonal antibody (Product # PA5-29569) in Western Blot

Wed Dec 21 00:00:00 EST 2022

Cardioids reveal self-organizing principles of human cardiogenesis.

Cell

Hofbauer P,Jahnel SM,Papai N,Giesshammer M,Deyett A,Schmidt C,Penc M,Tavernini K,Grdseloff N,Meledeth C,Ginistrelli LC,Ctortecka C,Šalic Š,Novatchkova M,Mendjan S

PA5-29569 was used in Immunocytochemistry to developmental heart chamber defects.

Thu Jun 10 00:00:00 EDT 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-29569 was used in Western Blotting to investigate the impact of peptidylarginine deiminase 4 on cardiac fibroblast function and myofibroblast transdifferentiation in vitro.

Fri Oct 01 00:00:00 EDT 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-29569 was used in Western Blotting to investigate the impact of peptidylarginine deiminase 4 on cardiac fibroblast function and myofibroblast transdifferentiation in vitro.

Fri Oct 01 00:00:00 EDT 2021

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

PA5-29569 was used in Western Blot to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 EDT 2021

An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair.

BioMed research international

Shao Z,Lyu C,Teng L,Xie X,Sun J,Zou D,Lu J

PA5-29569 was used in Immunohistochemistry to introduce some characteristics of IFS.

Tue May 25 00:00:00 EDT 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

Published figure using COL1A1 polyclonal antibody (Product # PA5-29569) in Western Blot

Mon May 10 00:00:00 EDT 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

Published figure using COL1A1 polyclonal antibody (Product # PA5-29569) in Western Blot

Mon May 10 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

PA5-29569 was used in Western Blotting and Immunocytochemistry to test if MSC-derived exosomes could accelerate wound healing by enhancing the biological properties of the main cell types involved in the key phases of this process.

Wed Jun 09 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Wed Jun 09 00:00:00 EDT 2021

Deficient Endoplasmic Reticulum Acetyl-CoA Import in Pancreatic Acinar Cells Leads to Chronic Pancreatitis.

Cellular and molecular gastroenterology and hepatology

Cooley MM,Thomas DDH,Deans K,Peng Y,Lugea A,Pandol SJ,Puglielli L,Groblewski GE

PA5-29569 was used in Immunocytochemistry to examine the role of the ER acetyl-CoA transporter AT-1 on pancreatic homeostasis.

Mon Dec 20 00:00:00 EST 2021

Small molecule inhibitor of HSP47 prevents pro-fibrotic mechanisms of fibroblasts in vitro.

Biochemical and biophysical research communications

Miyamura T,Sakamoto N,Kakugawa T,Taniguchi H,Akiyama Y,Okuno D,Moriyama S,Hara A,Kido T,Ishimoto H,Yamaguchi H,Miyazaki T,Obase Y,Ishimatsu Y,Tanaka Y,Mukae H

PA5-29569 was used in Western Blot to demonstrate that HSP47 is a potential therapeutic target for pulmonary fibrosis.

Thu Sep 24 00:00:00 EDT 2020

FOXM1 is a critical driver of lung fibroblast activation and fibrogenesis.

The Journal of clinical investigation

Penke LR,Speth JM,Dommeti VL,White ES,Bergin IL,Peters-Golden M

PA5-29569 was used in Western Blotting to identify FOXM1 as a driver of lung fibroblast activation and underscore the therapeutic potential of targeting FOXM1 for pulmonary fibrosis.

Fri Jun 01 00:00:00 EDT 2018

Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.

Biochemical and biophysical research communications

Qu B,Ma Y,Yan M,Gong K,Liang F,Deng S,Jiang K,Ma Z,Pan X

PA5-29569 was used in western blot to investigate the molecular mechanism of Sirt1 in osteogenic differentiation

Fri Sep 09 00:00:00 EDT 2016

MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.

Biochemical and biophysical research communications

Gong K,Qu B,Liao D,Liu D,Wang C,Zhou J,Pan X

PA5-29569 was used in western blot to elucidate mechanisms by which miR-132 regulates osteogenic differentiation

Fri Sep 09 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen I; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; Collagen1A1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

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Gene Aliases: Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4

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UniProt ID: (Human) P02452, (Rat) P02454, (Mouse) P11087

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Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842

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Function(s)

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding molecular_function extracellular matrix structural protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COL1A1 Polyclonal Antibody

COL1A1 Antibody (PA5-29569) in ICC/IF

Product Details

PA5-29569

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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