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COX2/ Cyclooxygenase 2 Polyclonal Antibody

COX2/ Cyclooxygenase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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COX2/ Cyclooxygenase 2 Antibody (27308-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human lung tissue slide using 27308-1-AP ( COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

COX2/ Cyclooxygenase 2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

COX2/ Cyclooxygenase 2 Antibody in Western Blot (WB)

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Product Details

27308-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 38 publications 38 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COX2/ Cyclooxygenase 2 Fusion Protein Ag26174 (377-604 aa encoded by BC013734)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LPDTFQIHD QKYNYQQFIY NNSILLEHGI TQFVESFTRQ IAGRVAGGRN VPPAVQKVSQ ASIDQSRQMK YQSFNEYRKR FMLKPYESFE ELTGEKEMSA ELEALYGDID AVELYPALLV EKPRPDAIFG ETMVEVGAPF SLKGLMGNVI CSPAYWKPST FGGEVGFQII NTASIQSLIC NNVKGCPFTS FSVPDPELIK TVTINASSSR SGLDDINPTV LLKERSTEL (377-604 aa encoded by BC013734)

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); TIS10 protein

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Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; Pghs-b; Pghs2; PHS-2; PTGS2; TIS10

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UniProt ID: (Human) P35354, (Mouse) Q05769

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Entrez Gene ID: (Human) 5743, (Mouse) 19225

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Function(s)

peroxidase activity prostaglandin-endoperoxide synthase activity protein binding lipid binding enzyme binding heme binding protein homodimerization activity metal ion binding arachidonate 15-lipoxygenase activity oxidoreductase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen dioxygenase activity oxygenase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COX2/ Cyclooxygenase 2 Polyclonal Antibody

COX2/ Cyclooxygenase 2 Antibody (27308-1-AP) in IHC (P)

Product Details

27308-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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