Invitrogen

COX4 Monoclonal Antibody (K.473.4)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

12 References

View all (43) COX4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

COX4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

COX4 Antibody (MA5-15078) in ICC/IF

Immunofluorescent analysis of COX IV in HeLa cells using a COX IV monoclonal antibody (Product # MA5-15078) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

COX4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

COX4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with COX4 siRNA and reduction of signal was observed in Western Blot using COX4 Monoclonal Antibody (K.473.4) (Product # MA5-15078). {KD}

Product Details

MA5-15078

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:800

View 1 publication 1 publication

Flow Cytometry (Flow)

1:400-1:1,600

Immunoprecipitation (IP)

1:100

Product Specifications

Species Reactivity

Non-human primate, Pig, Rat, Zebrafish, Bovine, Human

Published species

Human, Mouse, Non-human primate, Rat, Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.473.4

Immunogen

Synthetic peptide corresponding to residues surrounding Lys29 of human COX IV

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

331 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10987478

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4 (COX4I1) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brief Communication: Histological Assessment of Nonhuman Primate Brown Adipose Tissue Highlights the Importance of Sympathetic Innervation.

Journal of obesity

Williams AG,Long M,Kavanagh K

MA5-15078 was used in Immunohistochemistry to support the development of safe sympathetic nervous system stimulants to activate brown adipose tissue for obesity treatment.

Thu Feb 02 00:00:00 EST 2023

Brief Communication: Histological Assessment of Nonhuman Primate Brown Adipose Tissue Highlights the Importance of Sympathetic Innervation.

Journal of obesity

Williams AG,Long M,Kavanagh K

MA5-15078 was used in Immunohistochemistry to support the development of safe sympathetic nervous system stimulants to activate brown adipose tissue for obesity treatment.

Thu Feb 02 00:00:00 EST 2023

Brief Communication: Histological Assessment of Nonhuman Primate Brown Adipose Tissue Highlights the Importance of Sympathetic Innervation.

Journal of obesity

Williams AG,Long M,Kavanagh K

MA5-15078 was used in Immunohistochemistry to support the development of safe sympathetic nervous system stimulants to activate brown adipose tissue for obesity treatment.

Thu Feb 02 00:00:00 EST 2023

MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.

Redox biology

D'Onofrio N,Prattichizzo F,Martino E,Anastasio C,Mele L,La Grotta R,Sardu C,Ceriello A,Marfella R,Paolisso G,Balestrieri ML

MA5-15078 was used in Western Blotting to reveal for the first time that miR-27b could represent a possible target for future therapies aimed at improving endothelial health.

Thu Jun 01 00:00:00 EDT 2023

Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy.

Nature communications

Wang X,Zhang X,Cao K,Zeng M,Fu X,Zheng A,Zhang F,Gao F,Zou X,Li H,Li M,Lv W,Xu J,Long J,Zang W,Chen J,Gao F,Ding J,Liu J,Feng Z

MA5-15078 was used in Western Blotting to reveal a vital role for complex II assembly in the development of dilated cardiomyopathy and provide additional insights into therapeutic interventions for heart diseases.

Fri Jul 08 00:00:00 EDT 2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

The Journal of biological chemistry

Selinger M,Novotný R,Sýs J,Roby JA,Tykalová H,Ranjani GS,Vancová M,Jaklová K,Kaufman F,Bloom ME,Zdráhal Z,Grubhoffer L,Forwood JK,Hrabal R,Rumlová M,Štěrba J

MA5-15078 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show the first capsid protein 3D structure of a member of the tick-borne flaviviruses group and show that by metabolic labeling experiments, TBEVC and other flaviviral capsid proteins are able to induce translational shutoff and decrease of 18S rRNA.

Tue Nov 01 00:00:00 EDT 2022

On the potential role of globins in brown adipose tissue: a novel conceptual model and studies in myoglobin knockout mice.

American journal of physiology. Endocrinology and metabolism

Blackburn ML,Wankhade UD,Ono-Moore KD,Chintapalli SV,Fox R,Rutkowsky JM,Willis BJ,Tolentino T,Lloyd KCK,Adams SH

MA5-15078 was used in Western Blotting to suggest a role for myoglobin as an oxygen/nitric oxide-sensor that regulates cellular metabolic and signaling pathways.

Thu Jul 01 00:00:00 EDT 2021

Abnormal expression of lncRNA UCA1 disturbed cell apoptosis through mediating mitochondrial dynamics in PDAC.

Neoplasma

Teng B,Feng T,Li W,Wang Z

MA5-15078 was used in Western Blot to report that UCA1-driven change in mitochondrial dynamics induces mitochondrial apoptotic pathway in PDAC.

Mon Mar 01 00:00:00 EST 2021

Interspecies Differences in Proteome Turnover Kinetics Are Correlated With Life Spans and Energetic Demands.

Molecular & cellular proteomics : MCP

Swovick K,Firsanov D,Welle KA,Hryhorenko JR,Wise JP,George C,Sformo TL,Seluanov A,Gorbunova V,Ghaemmaghami S

MA5-15078 was used in Western Blot to suggest that in lieu of a rapid constitutive turnover, long-lived species may have evolved more energetically efficient mechanisms for selective detection and clearance of damaged proteins.

Fri Mar 11 00:00:00 EST 2022

Interspecies Differences in Proteome Turnover Kinetics Are Correlated With Life Spans and Energetic Demands.

Molecular & cellular proteomics : MCP

Swovick K,Firsanov D,Welle KA,Hryhorenko JR,Wise JP,George C,Sformo TL,Seluanov A,Gorbunova V,Ghaemmaghami S

Fri Mar 11 00:00:00 EST 2022

Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3.

International journal of molecular sciences

D'Onofrio N,Martino E,Mele L,Colloca A,Maione M,Cautela D,Castaldo D,Balestrieri ML

MA5-15078 was used in Western Blotting to support a critical role of PINK1/Parkin-mediated mitophagy in mitochondrial dysfunction and apoptosis induced by δVB in SW480 and SW620 colon cancer cells.

Thu Jul 29 00:00:00 EDT 2021

PARK14 PLA2G6 mutants are defective in preventing rotenone-induced mitochondrial dysfunction, ROS generation and activation of mitochondrial apoptotic pathway.

Oncotarget

Chiu CC,Yeh TH,Lu CS,Huang YC,Cheng YC,Huang YZ,Weng YH,Liu YC,Lai SC,Chen YL,Chen YJ,Chen CL,Chen HY,Lin YW,Wang HL

Published figure using COX4 monoclonal antibody (Product # MA5-15078) in Western Blot

Tue Oct 03 00:00:00 EDT 2017

A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.

Nature cell biology

Monaghan RM,Barnes RG,Fisher K,Andreou T,Rooney N,Poulin GB,Whitmarsh AJ

MA5-15078 was used in western blot to study the role of nuclear CLK-1 in regulating mitochondrial stress responses and aging.

Mon Jun 01 00:00:00 EDT 2015

A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.

Nature cell biology

Monaghan RM,Barnes RG,Fisher K,Andreou T,Rooney N,Poulin GB,Whitmarsh AJ

MA5-15078 was used in western blot to study the role of nuclear CLK-1 in regulating mitochondrial stress responses and aging.

Mon Jun 01 00:00:00 EDT 2015

3,5-Diiodo-L-thyronine activates brown adipose tissue thermogenesis in hypothyroid rats.

PloS one

Lombardi A,Senese R,De Matteis R,Busiello RA,Cioffi F,Goglia F,Lanni A

MA5-15078 was used in immunohistochemistry - paraffin section to test if 3,5-Diiodo-l-thyronine could activate brown adipose tissue thermogenesis

Fri Jan 15 00:00:00 EST 2016

Bioinformatics

Protein Aliases: cox iv; COX IV-1; Cox4-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; cytochrome c oxidase subunit IV precursor EC 1.9.3.1; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; cytochrome c oxydase subunit 4; FLJ23483; MGC72016

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Gene Aliases: COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; mg:bb02d03; zgc:110058

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UniProt ID: (Bovine) P00423, (Human) P13073, (Rat) P10888

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Entrez Gene ID: (Bovine) 281090, (Human) 1327, (Pig) 100624067, (Rat) 29445, (Zebrafish) 326975

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Function(s)

cytochrome-c oxidase activity protein binding oxidase

Process(es)

hydrogen ion transmembrane transport generation of precursor metabolites and energy mitochondrial electron transport, cytochrome c to oxygen response to nutrient response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COX4 Monoclonal Antibody (K.473.4)

COX4 Antibody (MA5-15078) in ICC/IF

Product Details

MA5-15078

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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