Proteintech

COX4I2 Polyclonal Antibody

COX4I2 Antibody in Immunocytochemistry (ICC/IF)

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COX4I2 Antibody (11463-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using COX4I2 antibody 11463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

COX4I2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COX4I2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11463-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COX4I2 Fusion Protein Ag1987 (1-171 aa encoded by BC057779)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was generated against full length COX4I2 protein.

Immunogen sequence: MLPRAAWSL VLRKGGGGRR GMHSSEGTTR GGGKMSPYTN CYAQRYYPMP EEPFCTELNA EEQALKEKEK GSWTQLTHAE KVALYRLQFN ETFAEMNRRS NEWKTVMGCV FFFIGFAALV IWWQRVYVFP PKPITLTDER KAQQLQRMLD MKVNPVQGLA SRWDYEKKQW KK (1-171 aa encoded by BC057779)

Target Information

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

COX4-1 promotes mitochondrial supercomplex assembly and limits reactive oxide species production in radioresistant GBM.

Cell stress

Oliva CR,Ali MY,Flor S,Griguer CE

Fri Apr 01 00:00:00 EDT 2022

Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.

Free radical biology & medicine

Ali MY,Oliva CR,Flor S,Goswami PC,Griguer CE

Fri May 20 00:00:00 EDT 2022

Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression.

Frontiers in oncology

Mao Y,Zhuo R,Ma W,Dai J,Alimu P,Fang C,Xu D,Ye L,Wang W,Sun F

Fri Sep 30 00:00:00 EDT 2022

Fibroblasts mediate the angiogenesis of pheochromocytoma by increasing COX4I2 expression.

Frontiers in oncology

Mao Y,Zhuo R,Ma W,Dai J,Alimu P,Fang C,Xu D,Ye L,Wang W,Sun F

Fri Sep 30 00:00:00 EDT 2022

Decreased mixed lineage leukemia 1 is involved in endometriosis-related infertility.

Journal of molecular endocrinology

Wen X,Xiong Y,Liu H,Geng T,Jin L,Zhang M,Ma L,Zhang Y

Fri Jan 01 00:00:00 EST 2021

Decreased mixed lineage leukemia 1 is involved in endometriosis-related infertility.

Journal of molecular endocrinology

Wen X,Xiong Y,Liu H,Geng T,Jin L,Zhang M,Ma L,Zhang Y

Fri Jan 01 00:00:00 EST 2021

COX4I2 is a novel biomarker of blood supply in adrenal tumors.

Translational andrology and urology

Mao Y,Ma W,Zhuo R,Ye L,Xu D,Wang W,Ning G,Sun F

Thu Jul 01 00:00:00 EDT 2021

Acute O₂ sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.

Science signaling

Moreno-Domínguez A,Ortega-Sáenz P,Gao L,Colinas O,García-Flores P,Bonilla-Henao V,Aragonés J,Hüttemann M,Grossman LI,Weissmann N,Sommer N,López-Barneo J

Tue Jan 21 00:00:00 EST 2020

Mitochondrial dysfunction in preclinical genetic prion disease: A target for preventive treatment?

Neurobiology of disease

Keller G,Binyamin O,Frid K,Saada A,Gabizon R

Mon Apr 01 00:00:00 EDT 2019

Mitochondrial dysfunction in preclinical genetic prion disease: A target for preventive treatment?

Neurobiology of disease

Keller G,Binyamin O,Frid K,Saada A,Gabizon R

Mon Apr 01 00:00:00 EDT 2019

Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.

Cell reports

Chowdhury A,Aich A,Jain G,Wozny K,Lüchtenborg C,Hartmann M,Bernhard O,Balleiniger M,Alfar EA,Zieseniss A,Toischer K,Guan K,Rizzoli SO,Brügger B,Fischer A,Katschinski DM,Rehling P,Dudek J

Tue Oct 16 00:00:00 EDT 2018

Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.

Cell reports

Chowdhury A,Aich A,Jain G,Wozny K,Lüchtenborg C,Hartmann M,Bernhard O,Balleiniger M,Alfar EA,Zieseniss A,Toischer K,Guan K,Rizzoli SO,Brügger B,Fischer A,Katschinski DM,Rehling P,Dudek J

Tue Oct 16 00:00:00 EDT 2018

Gene expression analyses reveal metabolic specifications in acute O₂ -sensing chemoreceptor cells.

The Journal of physiology

Gao L,Bonilla-Henao V,García-Flores P,Arias-Mayenco I,Ortega-Sáenz P,López-Barneo J

Fri Sep 15 00:00:00 EDT 2017

Gene expression analyses reveal metabolic specifications in acute O₂ -sensing chemoreceptor cells.

The Journal of physiology

Gao L,Bonilla-Henao V,García-Flores P,Arias-Mayenco I,Ortega-Sáenz P,López-Barneo J

Fri Sep 15 00:00:00 EDT 2017

Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.

Biochimica et biophysica acta. Molecular cell research

Aras S,Arrabi H,Purandare N,Hüttemann M,Kamholz J,Züchner S,Grossman LI

Wed Feb 01 00:00:00 EST 2017

Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.

Biochimica et biophysica acta. Molecular cell research

Aras S,Arrabi H,Purandare N,Hüttemann M,Kamholz J,Züchner S,Grossman LI

Wed Feb 01 00:00:00 EST 2017

Repositioning chlorpromazine for treating chemoresistant glioma through the inhibition of cytochrome c oxidase bearing the COX4-1 regulatory subunit.

Oncotarget

Oliva CR,Zhang W,Langford C,Suto MJ,Griguer CE

Tue Jun 06 00:00:00 EDT 2017

Repositioning chlorpromazine for treating chemoresistant glioma through the inhibition of cytochrome c oxidase bearing the COX4-1 regulatory subunit.

Oncotarget

Oliva CR,Zhang W,Langford C,Suto MJ,Griguer CE

Tue Jun 06 00:00:00 EDT 2017

Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.

Oncotarget

Oliva CR,Markert T,Gillespie GY,Griguer CE

Sat Feb 28 00:00:00 EST 2015

Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.

Oncotarget

Oliva CR,Markert T,Gillespie GY,Griguer CE

Sat Feb 28 00:00:00 EST 2015

GnRH-Gemcitabine conjugates for the treatment of androgen-independent prostate cancer: pharmacokinetic enhancements combined with targeted drug delivery.

Bioconjugate chemistry

Karampelas T,Argyros O,Sayyad N,Spyridaki K,Pappas C,Morgan K,Kolios G,Millar RP,Liapakis G,Tzakos AG,Fokas D,Tamvakopoulos C

Wed Apr 16 00:00:00 EDT 2014

GnRH-Gemcitabine conjugates for the treatment of androgen-independent prostate cancer: pharmacokinetic enhancements combined with targeted drug delivery.

Bioconjugate chemistry

Karampelas T,Argyros O,Sayyad N,Spyridaki K,Pappas C,Morgan K,Kolios G,Millar RP,Liapakis G,Tzakos AG,Fokas D,Tamvakopoulos C

Wed Apr 16 00:00:00 EDT 2014

Type II diabetes increases mitochondrial DNA mutations in the left ventricle of the Goto-Kakizaki diabetic rat.

American journal of physiology. Heart and circulatory physiology

Hicks S,Labinskyy N,Piteo B,Laurent D,Mathew JE,Gupte SA,Edwards JG

Mon Apr 01 00:00:00 EDT 2013

Type II diabetes increases mitochondrial DNA mutations in the left ventricle of the Goto-Kakizaki diabetic rat.

American journal of physiology. Heart and circulatory physiology

Hicks S,Labinskyy N,Piteo B,Laurent D,Mathew JE,Gupte SA,Edwards JG

Mon Apr 01 00:00:00 EDT 2013

Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.

Free radical biology & medicine

Schneider L,Giordano S,Zelickson BR,S Johnson M,A Benavides G,Ouyang X,Fineberg N,Darley-Usmar VM,Zhang J

Thu Dec 01 00:00:00 EST 2011

Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.

Free radical biology & medicine

Schneider L,Giordano S,Zelickson BR,S Johnson M,A Benavides G,Ouyang X,Fineberg N,Darley-Usmar VM,Zhang J

Thu Dec 01 00:00:00 EST 2011

Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain.

The Journal of biological chemistry

Oliva CR,Nozell SE,Diers A,McClugage SG,Sarkaria JN,Markert JM,Darley-Usmar VM,Bailey SM,Gillespie GY,Landar A,Griguer CE

Fri Dec 17 00:00:00 EST 2010

Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain.

The Journal of biological chemistry

Oliva CR,Nozell SE,Diers A,McClugage SG,Sarkaria JN,Markert JM,Darley-Usmar VM,Bailey SM,Gillespie GY,Landar A,Griguer CE

Fri Dec 17 00:00:00 EST 2010

Bioinformatics

Protein Aliases: COX IV-2; Cytochrome c oxidase subunit 4 isoform 2, mitochondrial; Cytochrome c oxidase subunit IV isoform 2; cytochrome c oxidase subunit IV isoform 2 (lung); cytochrome c oxidase subunit IV-like 2; cytochrome c oxidase, subunit 4b; cytochrome c oxidase, subunit IVb

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Gene Aliases: COX4; COX4-2; COX4B; COX4I2; COX4L2; COXIV-2; dJ857M17.2

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UniProt ID: (Human) Q96KJ9, (Mouse) Q91W29, (Rat) Q91Y94

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Entrez Gene ID: (Human) 84701, (Mouse) 84682, (Rat) 84683

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Function(s)

cytochrome-c oxidase activity oxidase

Process(es)

generation of precursor metabolites and energy mitochondrial electron transport, cytochrome c to oxygen cellular respiration oxidation-reduction process hydrogen ion transmembrane transport biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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COX4I2 Polyclonal Antibody

COX4I2 Antibody (11463-1-AP) in ICC/IF

Product Details

11463-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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