Proteintech

CPS1 Polyclonal Antibody

CPS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

CPS1 Antibody (18703-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (Product # 18703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CPS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

18703-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.

Target Information

The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

p53 inhibits the Urea cycle and represses polyamine biosynthesis in glioma cell lines.

Metabolic brain disease

Zhao Y,Chen Y,Wei L,Ran J,Wang K,Zhu S,Liu Q

Sat Apr 01 00:00:00 EDT 2023

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 EDT 2023

Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.

Frontiers in immunology

Peng SL,Wang R,Zhou YL,Wei W,Zhong GH,Huang XT,Yang S,Liu QD,Liu ZG

Tue Jul 12 00:00:00 EDT 2022

Serum proteome profiling provides a deep understanding of the 'gut-liver axis' in relation to liver injury and regeneration.

Acta biochimica et biophysica Sinica

Leng L,Ma J,Lv L,Gao D,Li M,Wang Y,Zhu Y

Tue Mar 02 00:00:00 EST 2021

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Frontiers in cell and developmental biology

Bai C,Wang H,Dong D,Li T,Yu Z,Guo J,Zhou W,Li D,Yan R,Wang L,Wang Z,Li Y,Ren L

Wed Apr 21 00:00:00 EDT 2021

Plasmodium sporozoite phospholipid scramblase interacts with mammalian carbamoyl-phosphate synthetase 1 to infect hepatocytes.

Nature communications

Cha SJ,Kim MS,Na CH,Jacobs-Lorena M

Fri Nov 19 00:00:00 EST 2021

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Biomolecules

Ceccacci S,Deitersen J,Mozzicafreddo M,Morretta E,Proksch P,Wesselborg S,Stork B,Monti MC

Tue Jun 02 00:00:00 EDT 2020

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Molecular metabolism

Zhang W,Dong Z,Xu M,Zhang S,Liu C,Chen S

Sat Feb 01 00:00:00 EST 2020

Therapeutic effect of N-carbamylglutamate in CPS1 deficiency.

Molecular genetics and metabolism reports

Sugiyama Y,Shimura M,Ogawa-Tominaga M,Ebihara T,Kinouchi Y,Isozaki K,Matsuhashi T,Tajika M,Fushimi T,Ichimoto K,Matsunaga A,Ishida T,Mizutani K,Tsuruoka T,Murayama K

Tue Sep 01 00:00:00 EDT 2020

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Journal of proteome research

Cao Y,Ding W,Zhang J,Gao Q,Yang H,Cao W,Wang Z,Fang L,Du R

Fri May 03 00:00:00 EDT 2019

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Journal of proteome research

Cao Y,Ding W,Zhang J,Gao Q,Yang H,Cao W,Wang Z,Fang L,Du R

Fri May 03 00:00:00 EDT 2019

Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells.

Journal of cellular and molecular medicine

Liu X,Zhang X,Bi J,Li Z,Zhang Z,Kong C

Sun Dec 01 00:00:00 EST 2019

Studies on the biological functions of CPS1 in AFB1 induced hepatocarcinogenesis.

Gene

Yang C,Fu R,Zhuang Z,Wang S

Mon Oct 10 00:00:00 EDT 2016

Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage.

Cancer cell

Tummala KS,Gomes AL,Yilmaz M,Graña O,Bakiri L,Ruppen I,Ximénez-Embún P,Sheshappanavar V,Rodriguez-Justo M,Pisano DG,Wagner EF,Djouder N

Mon Dec 08 00:00:00 EST 2014

Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage.

Cancer cell

Tummala KS,Gomes AL,Yilmaz M,Graña O,Bakiri L,Ruppen I,Ximénez-Embún P,Sheshappanavar V,Rodriguez-Justo M,Pisano DG,Wagner EF,Djouder N

Mon Dec 08 00:00:00 EST 2014

Proteomics analysis of human nonalcoholic fatty liver.

Methods in molecular biology (Clifton, N.J.)

Rodriguez-Suarez E,Mato JM,Elortza F

Mon Dec 17 00:00:00 EST 2012

Proteomics analysis of human nonalcoholic fatty liver.

Methods in molecular biology (Clifton, N.J.)

Rodriguez-Suarez E,Mato JM,Elortza F

Mon Dec 17 00:00:00 EST 2012

Non-alcoholic fatty liver disease proteomics.

Proteomics. Clinical applications

Rodríguez-Suárez E,Duce AM,Caballería J,Martínez Arrieta F,Fernández E,Gómara C,Alkorta N,Ariz U,Martínez-Chantar ML,Lu SC,Elortza F,Mato JM

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: carbamoyl-phosphate synthase (ammonia); carbamoyl-phosphate synthase 1, mitochondrial; Carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthetase 1, mitochondrial; Carbamoyl-phosphate synthetase I; carbamoylphosphate synthetase I; carbamyl phosphate synthetase I; carboamyl-phosphate synthetase 1; CPSase I

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Gene Aliases: 4732433M03Rik; CPS; CPS1; CPSASE1; D1Ucla3; PHN

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UniProt ID: (Human) P31327, (Mouse) Q8C196, (Rat) P07756

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Entrez Gene ID: (Human) 1373, (Mouse) 227231, (Rat) 497840

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Function(s)

aspartate carbamoyltransferase activity carbamoyl-phosphate synthase (ammonia) activity carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity dihydroorotase activity endopeptidase activity calcium ion binding protein binding ATP binding phospholipid binding glutamate binding protein complex binding modified amino acid binding nucleotide binding catalytic activity ligase activity metal ion binding ligase

Process(es)

urea cycle glycogen catabolic process 'de novo' pyrimidine nucleobase biosynthetic process proteolysis arginine biosynthetic process glutamine metabolic process midgut development response to toxic substance response to zinc ion response to amine citrulline biosynthetic process triglyceride catabolic process response to food response to lipopolysaccharide response to drug response to starvation response to amino acid cellular response to fibroblast growth factor stimulus positive regulation of vasodilation nitric oxide metabolic process homocysteine metabolic process anion homeostasis response to growth hormone hepatocyte differentiation carbamoyl phosphate biosynthetic process cellular response to cAMP cellular response to glucagon stimulus cellular response to oleic acid response to dexamethasone nitrogen compound metabolic process metabolic process liver development glutamine catabolic process response to glucagon response to oleic acid response to steroid hormone response to glucocorticoid response to cAMP

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CPS1 Polyclonal Antibody

CPS1 Antibody (18703-1-AP) in ICC/IF

Product Details

18703-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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