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CPT1A Antibody (MA5-51274) in WB
Western blot analysis of CPT1A in MCF7 cells. Samples were incubated with CPT1A Recombinant monoclonal antibody (Product # MA5-51274) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
MA5-51274
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3M6E9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093121
Product Specific Information
Immunogen sequence: WDIPGECQEV IETSLNTANL LANDVDFHSF PFVAFGKGII KKCRTSPDAF VQLALQLAHY KDMGKFCLTY EASMTRLFRE GRTETVRSCT TESCDFVRAM VDPAQTVEQR LKLFKLASEK HQHMYRLAMT GSGIDRHLFC LYVVSKYLAV ESPFLKEVLS EPWRLSTSQT PQQQVELFDL ENNPEYVSSG GGFGPVADDG Y
Target Information
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT 1; CPT I; CPT1 L; CPT1-L; CPTI-L; Succinyltransferase CPT1A
Gene Aliases: CPT1; CPT1-L; CPT1A; L-CPT1
UniProt ID: (Human) P50416
Entrez Gene ID: (Human) 1374
long-chain fatty acid metabolic process
glucose metabolic process
fatty acid beta-oxidation
triglyceride metabolic process
carnitine shuttle
circadian rhythm
carnitine metabolic process
response to organic cyclic compound
epithelial cell differentiation
positive regulation of fatty acid beta-oxidation
response to drug
eating behavior
regulation of insulin secretion
protein homooligomerization
cellular response to fatty acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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